非CO_2依赖型细胞培养系统对前列腺癌PC-3细胞增殖的影响  

Effects of CO_2-independent cell culture system on the proliferation of prostate carcinoma cell line PC-3

作　　者：张琳刚[1] 张天禹[1] 谭宁[2] 

机构地区：[1]桂林医学院附属医院泌尿外科,广西桂林541001 [2]桂林医学院生物技术学院,广西桂林541001

出　　处：《西安交通大学学报（医学版）》2010年第5期612-616,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：广西壮族自治区卫生厅医疗卫生重点科研课题(No.重200982);广西壮族自治区卫生厅科研课题(No.Z2007320);科技部癌基因及相关基因国家重点实验室开放课题(No.90-08-01)~~

摘　　要：目的探讨非CO2依赖型细胞培养系统对前列腺癌PC-3细胞增殖的影响。方法对前列腺癌PC-3细胞采取非CO2依赖型细胞培养系统及CO2依赖型细胞培养系统进行培养,均在37℃培养箱(大气环境)和37℃,50 mL/LCO2培养箱条件下,检测分析两种体系pH的变化;采用细胞增殖曲线及克隆形成实验检测细胞生长差异情况;并使用RT-PCR技术检测分析两种体系下的前列腺癌细胞中survivin、caspase-3基因的表达差异。结果两种培养系统条件下,细胞培养基pH变化趋势一致(P>0.05);两种培养系统下PC-3细胞生长增殖未见差异(P>0.05);RT-PCR法检测显示两种培养系统下表达survivin、caspase-3未见差异(P>0.05)。结论前列腺癌非CO2依赖型细胞培养系统对细胞增殖、基因表达等方面与前列腺癌CO2依赖型细胞培养系统无显著差异。Objective To evaluate the effects of CO2-independent culture cell system on the proliferation of prostate carcinoma cell line PC-3.Methods Prostate carcinoma cell line PC-3 was cultured using CO2-independent culture cell system and CO2-dependent culture cell system at 37 ℃ in 50 mL/L carbon dioxide incubator and at 37 ℃ in air incubator.The pH of culture medium with the two culture cell systems was detected.Differences in cell viability were assayed with CCK-8 test and colony forming assay.The expressions of survivin and caspase-3 mRNA of cell culturing with the two systems were detected by RT-PCR assay.Results Under the two culture cell systems,the pH trend was the same（P〈0.05）,PC-3 cell proliferation and the expressions of survivin and caspase-3 mRNA of the cell had no obvious differences（P〈0.05）.Conclusion CO2-independent culture cell system is parallel to CO2-dependent culture cell system in their effects on cell proliferation and gene expression.

关 键 词：PC-3前列腺癌细胞 非CO2依赖 SURVIVIN CASPASE-3 

分 类 号：R737.25[医药卫生—肿瘤]