Darbufelone对胃癌SGC-7901细胞侵袭转移能力的影响  

Inhibition of gastric cancer cells invasion and metastasis by Darbufelone in vitro

作　　者：石建华[1] 刘纯伦[1] 罗访[1] 潘敏[1] 徐晓萌[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016

出　　处：《重庆医科大学学报》2010年第7期1021-1025,共5页Journal of Chongqing Medical University

基　　金：重庆市卫生局课题(编号:05-2-155)

摘　　要：目的:观察环氧合酶-2/5-脂氧合酶(COX-2/5-LOX)双重抑制剂Darbufelone对人胃癌SGC-7901细胞异质粘附、侵袭、迁移、血管形成能力的影响,并初步探讨其可能机制。方法:用Darbufelone处理SGC-7901细胞,MTT法检测其粘附能力的变化;Transwell Chamber膜侵袭系统观察其侵袭和迁移能力的改变;观察SGC-7901细胞条件培养基诱导内皮细胞形成血管管腔能力;RT-PCR和免疫细胞化学分析SGC-7901细胞中骨桥蛋白(Osteopontin,OPN)和基质金属蛋白酶-2(Matrix metalloproteinases-2,MMP-2)的mRNA与蛋白表达变化。结果:经1.5×10-5mol/L的Darbufelone处理后,SGC-7901细胞粘贴率明显降低(P<0.05),游走穿膜细胞数(93.00±6.56)、侵袭穿膜细胞数(50.00±3.67)均显著低于对照组(143.00±9.82,82.00±7.14,P<0.01);诱导内皮细胞形成血管管腔数(82.70±4.58)与管腔长度([172.20±6.41)μm]均较对照组[142.00±5.86,(214.60±9.44)μm]显著减少(P<0.01);OPN和MMP-2的mRNA表达(0.34±0.03、0.33±0.04)与蛋白表达(0.18±0.01、0.26±0.02)分别较对照组的mRNA表达(0.54±0.01、0.57±0.09)和蛋白表达(0.26±0.03、0.33±0.03)明显降低(P<0.05)。结论:COX-2/5-LOX双重抑制剂Darbufelone能有效抑制SGC-7901细胞的侵袭转移,其作用机制可能与抑制OPN和MMP-2的表达有关。Objective：To study the effects of Darbufelone on the invasion and metastasis of human gastric cancer cells SGC-7901 in vitro. Methods：The adhesion ability was determined by MTT.Invasion and metastasis was observed in Transwell Chamber membrane invasion culture system.The capability of SGC-7901 cell condition medium in inducing endothelial cell to form blood vessel in 24 well plate was detected.RT-PCR and cel1-immunohistochemistry were used to observe the expressions of OPN and MMP-2.Results：In the membrane invasion culture system,the numbers of invading and migrating SGC-7901 cells were significantly lower in Darbufelone treated groups compared with those in the control group（sP0.05）.The vessel number and the length of vessel were significantly lower in Darbufelone treated groups compared with those in the control group（sP0.05）.The adhesion rate,OPN and MMP-2 expression were down regulated in Darbufelone group（sP0.05）.Conclusion：Darbufelone can inhibit the migration and invasion of SGC-7901 cells in vitro, probably via down-regulation of OPN and MMP-2.

关 键 词：SGC-7901细胞 环氧合酶-2 5-脂氧合酶 侵袭 转移 

分 类 号：R735.2[医药卫生—肿瘤]