p16^(INK4a)在子宫颈细胞学分级的临床意义  

The clinical significance of p16^(INK4a) in cervical cytology staging

作　　者：黄萱[1] 陈莹[1] 陈琼霞[1] 邓昊[1] 王成鑫[2] 刘丽江[2] 

机构地区：[1]江汉大学医学院,武汉430056 [2]江汉大学病理诊断所,武汉430056

出　　处：《重庆医科大学学报》2010年第7期1026-1028,共3页Journal of Chongqing Medical University

摘　　要：目的:研究在意义不明的不典型鳞状细胞(Atypical squamous cells of undetermined significance,ASC-US),不能排除高度上皮内瘤变的不典型鳞状细胞(Atypical squamous cells,cannot exclude high-grade squamous intraepithelial lesion,ASC-H),低度鳞状上皮内病变(Low-grade squamous intraepithelial lesion,LSIL),高度鳞状上皮内病变(High-grade squamous intraepithelial lesion,HSIL)中p16INK4a蛋白和HPV16/18的表达水平及意义。方法:应用免疫组织化学技术和原位分子杂交技术,对89例子宫颈细胞涂片样本中p16INK4a蛋白及HPV16/18型DNA进行检测。结果:p16INK4a蛋白阳性率随着子宫颈病变程度的加重而升高,LSIL、HSIL及ASC-H的p16INK4a蛋白阳性率均高于ASC-US(P<0.05);ASC-US和LSIL的p16INK4a阳性表达与HPV16/18阳性表达无差异性;LSIL和ASC-US的HPV16/18阳性表达无差异性。结论:子宫颈细胞涂片样本中p16INK4a蛋白检测能协助子宫颈细胞学诊断分级,尤其对于ASC-US的病例,需同时做p16INK4a免疫组织化学检查,以帮助临床医生选择恰当的处理方式。Objective：To investigate the expression and significance ofp16INK4a and HPV16/18 in atypical squamous cells of undetermined significance（ASC-US）,atypical squamous cells,cannot exclude high-grade squamous intraepithelial lesion（ASC-H）,low-grade squamous intraepithelial lesion（LSIL）,high-grade squamous intraepithelial lesion（HSIL）.Methods：p16INK4a and HPV16/18 DNA in thin-layer slides from 89 specimens were detected by immunohistochemistry and the insitu hybridization.Results：A positive correlation was found between the positive rate of p16INK4a and cervical disease progression,the positive rate of p16INK4a in LSIL,HSIL,ASC-H were higher than ASC-US（P0.05）;There were not variability between the positive rate of p16INK4a and HPV16/18 DNA in ASC-US and LSIL; p16INK4a positive rate in ASC-US was not related with p16INK4a positive rate in LSIL.Conclusion：Detecting p16INK4a in thin-layer slides could help staging in cervical cytology,p16INK4a should be detected by immunohistochemistry for ASC-US,which could help clinical doctor choose a curative treatment.

关 键 词：P16INK4A HPV16/18型 子宫颈细胞学 

分 类 号：R36[医药卫生—病理学]