手霉素对人卵巢癌3AO细胞增殖和凋亡的影响  

Effects of manumycin on proliferation and apoptosis of human ovarian cancer 3AO cells

作　　者：张正茂[1] 顾来梅 刘洁[1] 张超[2] 张风华[3] 康山 单保恩[2] 王士杰[2] 

机构地区：[1]河北医科大学第四医院妇产科 [2]河北医科大学第四医院科研中心,石家庄050011 [3]河北省人民医院外科,石家庄050026

出　　处：《肿瘤》2010年第8期651-655,共5页Tumor

基　　金：河北省教育厅普通高校强势特色学科资助项目[编号:冀教高(2005)52号]

摘　　要：目的:探讨手霉素对人卵巢癌3AO细胞体外增殖和凋亡的影响及其可能的作用机制。方法:MTT法检测手霉素对3AO细胞的增殖抑制作用;Giemsa染色、透射电子显微镜下观察及FCM分析手霉素对3AO细胞的凋亡诱导作用;FCM检测手霉素对3AO细胞ras、survivin和核因子-κB(nuclear factor-kappa B,NF-κB)(p65)蛋白表达的影响。结果:手霉素能显著抑制3AO细胞的增殖(P<0.05),并且这种抑制作用随着手霉素浓度的增加和作用时间的延长而增强。Giemsa染色和透射电子显微镜下观察发现,手霉素可诱导3AO细胞出现典型的凋亡形态学变化及超微结构改变。FCM分析结果显示,与对照组相比,手霉素诱导3AO细胞凋亡率明显增加(P<0.05),细胞周期中的G2/M期细胞也明显增加(P<0.05);3AO细胞的ras、NF-κB(p65)和survivin蛋白的表达水平明显下降(P<0.05)。结论:手霉素可明显抑制人卵巢癌3AO细胞的增殖并诱导其凋亡,其作用机制可能与下调细胞ras、NF-κB(p65)和survivin蛋白的表达有关。Objective ：To study the effects of manumycin on proliferation and apoptosis of human ovarian cancer 3AO cells, and elucidate the possible mechanisms. Methods ： MTT colorimetric method was performed to evaluate the cytotoxic effects of manumycin on 3AO cells. The apoptosis of 3AO cells was observed by Giemsa staining, transmission electron microscopy, and flow cytometry techniques. Expressions of ras, survivin and nuclear factor-kappa B （NF-κB） （p65） proteins of 3AO cells induced by manumycin were analyzed by flow cytometry. Results：Manumycin significantly inhibited the proliferation of 3AO cells in a dose-and time-dependent way （P 〈 0.05 ）. The typical apoptotic morphological and super-microstructural changes were observed by light microscopy and transmission electron microscopy after manumycin treatment. Compared with control group, the apoptosis rate and the percentage of 3AO cells in G2/M phase were significantly increased in manumycin treatment group （ P 〈 0.05 ）. Expressions of ras, NF-κB （ p65 ） and survivin protein in 3AO cells treated with manumycin were down-regulated. Conclusion： Manumycin inhibits the proliferation of human ovarian cancer 3AO cells and induced the apoptosis. The mechanism may be associated with down-regulation of the expression of ras, NF-κB （ p65 ） , and survivin protein.

关 键 词：卵巢肿瘤 基因表达 细胞凋亡 手霉素 细胞 3AO 

分 类 号：R737.31[医药卫生—肿瘤]