肠杆菌科AcrAB多药外排泵分子演化分析  

Molecular Evolutionary Analysis of Enterobacteriaceae AcrAB Multidrug Efflux Pump

作　　者：侯进慧[1] 陈宏伟[1] 高兆建[1] 蔡侃[1] 

机构地区：[1]徐州工程学院,江苏徐州221008

出　　处：《生物技术》2010年第4期18-21,共4页Biotechnology

基　　金：徐州市科技计划项目(XZZD0924);徐州工程学院引进人才科研启动项目;徐州工程学院科研项目(XKY2008110)资助

摘　　要：目的:分析肠杆菌科AcrAB多药外排泵的分子演化规律,为多药耐药性病原菌的防治提供基础数据。方法:从NCBI获得肠杆菌科物种AcrAB多药外排泵相关蛋白和核酸序列,采用分析软件,分析肠杆菌科物种AcrAB多药外排泵相关序列。结果:在肠杆菌科各物种AcrA、AcrB和AcrR与大肠埃希氏菌同源性在55%、75%和43%以上。AcrA保守位点分散,在N端和C端较少,在分子一级结构中段较多。AcrB跨膜序列保守性较高,与质子转移相关的三个位点D407、D408和K940以及稳定这三者结构的T978在肠杆菌科完全保守,其一级结构上相邻位点也保守。AcrR序列整体保守性较低,但HTH区域保守性高。与AcrR结合的回文结构及周围序列保守性高,在"茎"结构中仅存在一个氨基酸的差异。结论:AcrAB多药外排泵在肠杆菌科中广泛存在,有一定的保守性。分析肠杆菌科AcrAB多药外排泵有助于病原菌多药耐药性的防治。Objective：To analyze molecular evolution of Enterobacteriaceae AcrAB multidrug efflux pump,and get primary data for the treatment of multidrug resistance pathogens.Method：NCBI data of protein and nuclear sequences related to Enterobacteriaceae AcrAB multidrug efflux pump were analyzed with protein and nucleotide multiple sequence alignment software.Result：Compared with Escherichia coli,homologous of AcrA,AcrB and AcrR in Enterobacteriaceae are more than 55% ,75% and 43% respectively.AcrA conservative sites are dispersive,with less conservative sequences in N and C terminal and more in middle sequence of primary structure.Transmembrane sequences of AcrB are conservative.Putative proton translocation related sites,D407,D408 and K940 and T978 which acts as a stabilizer between the former three sites are completely conservative,and their flanking sequences are also conservative.AcrR sequence as a whole is less conservative,but its HTH domain is highly conservative.AcrR-binding palindrome and flanking sequence are highly conservative and there is only one site in the middle of stem structure are variable.Conclusion：AcrAB multidrug efflux pump gene generally exists in Enterobacteriaceae and is conservative.Analysis of Enterobacteriaceae AcrAB multidrug efflux pump will promote treatment of pathogen multidrug resistance.

关 键 词：肠杆菌科 AcrAB 多药外排泵 分子演化 

分 类 号：Q93-331[生物学—微生物学]