复方中药制剂对具核梭杆菌抗菌作用的体外研究  

In vitro antibacterial effects of a traditional Chinese medicine compound on Fusobacterium nucleatum

作　　者：黄莉莉[1] 马瑞[1] 夏文薇[1] 朱彩莲[1] 叶冬霞[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院.牙体牙髓病科上海市口腔医学重点实验室,上海200011

出　　处：《牙体牙髓牙周病学杂志》2010年第8期437-440,共4页Chinese Journal of Conservative Dentistry

基　　金：上海市卫生局中医处资助项目(2008L008A);上海市科学技术委员会资助项目(08DZ2271100);上海市重点学科建设项目资助(S30206)

摘　　要：目的:观察由没食子酸、厚朴酚和白芨多糖三者配伍制成的复方中药制剂对具核梭杆菌的抗菌作用。方法:采用液体二倍稀释法,测定复方中药制剂对游离状态下具核梭杆菌的最小抑菌浓度和最小杀菌浓度;在体外形成生物膜后,分别用复方中药制剂和氧化锌液作用30min,利用激光共聚焦扫描显微镜观察生物膜的细菌活性。结果:复方中药制剂、氧化锌液对游离状态下具核梭杆菌均有一定的抗菌作用,但两者的最小抑菌、杀菌浓度无显著性差异(P>0.05);通过对具核梭杆菌生物膜的观察,发现在使用中药制剂后,具核梭杆菌生物膜活菌百分比显著下降,与氧化锌、空白对照组相比均有显著性差异(P<0.05)。结论:复方中药制剂对具核梭杆菌具有较强的抗菌作用,尤其对生物膜状态下的具核梭杆菌,抗菌作用更明显。AIM：To assess the antibacterial effect of a traditional Chinese medicine compound（CTM）,composed of gallic acid,magnolol and polysaccharide of Blettila striata,on F.nucleatum in vitro.METHODS：The minimal inhibitory concentration（MIC） and minimal bactericidal concentration（MBC） of CTM and zinc oxide against F.nucleatum were measured by the broth dilution technique.Biofilms formed by F.nucleatum were observed under a confocal laser scan microscope（CLSM）.RESULTS：CTM and zinc oxide showed similar MIC and MBC on F.nucleatum（P 0.05）.Compared with blank control,CTM and zinc oxide treatment resulted in a significant decrease of vital bacterial cells as observed by CLSM（P 0.05）.CONCLUSION：The traditional Chinese medicine compound is an effective antibacterial agent against F.nucleatum in vitro.Its effects are greater than zinc oxide on F.nucleatum biofilm.

关 键 词：复方中药 具核梭杆菌 生物膜 根管封闭剂 

分 类 号：R780.2[医药卫生—口腔医学]