心肺复苏后大鼠神经细胞Caspase-3表达与细胞凋亡关系的实验研究  

The relationship between expression of Caspase - 3 and apoptosis of neuron post cardiopulmonary resuscitation in rats

作　　者：马宇洁[1] 杨兴易[2] 林兆奋[1] 缪明永[3] 宁波[1] 

机构地区：[1]空军总医院重症监护科,北京100142 [2]解放军第二军医大学附属长征医院急救科 [3]解放军第二军医大学基础部生物化学与分子生物学线粒体功能室

出　　处：《中国急救医学》2010年第8期708-710,共3页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金资助项目（No.30500173）

摘　　要：目的探讨心肺复苏后大鼠脑组织Caspase-3表达与细胞凋亡的关系。方法建立窒息联合冰氯化钾致大鼠心脏骤停／心肺复苏（CA／CPR）的动物模型，于各时相点断头留取脑组织，采用免疫组化的方法观察CPR后脑组织Caspase-3蛋白的表达，TUNEL检测神经细胞凋亡。结果对照组脑组织内仅有少量TUNEL阳性细胞；ROSC后3h出现表达阳性细胞，ROSC后6h表达开始增多，ROSC后12h进一步上升（P〈0．05），其后各组均处于高水平表达。Caspase-3表达均在ROSC后24h显著增加，直至ROSC后72h，与对照组比较差异均有统计学意义（P〈0．01），Speamaa等级相关检验Caspase-3表达与细胞凋亡明显相关（r=0．909）。结论CA／CPR后大鼠脑线粒体Caspase-3蛋白高表达可能是细胞凋亡增加的原因之一。Objective To study the the relationship between expression of Caspase - 3 and apoptosis of neuron post cardiopulmonary resuscitation in rats. Methods Sprague Dawley rats cardiac arrest was induced by asphyxiation and ice - cold in 5 minutes of no circulation. 56 male Sprague Dawley rats were randomly divided into 7 groups ： sham control group （ n = 8 ） ; after cardiopulmonary resuscitation the rats were allowed to reperfuse spontaneously for 3 h, 6 h, 12 h, 24 h, 48 h, 72 h, with each group of 8 rats. The rats were killed by decapitation and the brains were removed. The expression of Caspase - 3 and apoptosis of neuron in hippocampus of the model rats was observed by means of the immuhistochemistry and TUNEL respectively. Results Typical cell apoptosis was induced in neuron at 3 h and significant increase at 12 h post restoration of spontaneous circulation（ROSC） . Upregulation of Caspase - 3 expressions was maintained during 24 h post ROSC. Expression of Caspase - 3 in neuron was in accordance with the account of the apoptosis neuron. Conclusion Cardiac arrest and eardiopulmonary resuscitation induce the apeptosis of neuron, high expression of Caspase - 3 may one of the mechanism.

关 键 词：大鼠 线粒体 半胱天冬蛋白酶-3 心脏骤停 心肺复苏 凋亡 

分 类 号：R651.15[医药卫生—外科学]