肿瘤特异性细胞毒T细胞对B16细胞的杀伤作用  

THE KILLING EFFECTS OF TUMOR SPECIFIC CYTOTOXIC T CELLS ON B16 CELLS

作　　者：苗乃法[1,2] 王艳华[1,2] 冯永堂[1,2] 鞠吉雨 郝桂兰[1,2] 吴强 李在连[1,2] 徐长夏 

机构地区：[1]潍坊医学院免疫学教研室 [2]青岛卫生学校

出　　处：《潍坊医学院学报》1999年第1期26-27,共2页Acta Academiae Medicinae Weifang

摘　　要：目的研究肿瘤特异性细胞毒Ｔ细胞对其靶细胞的杀伤作用。方法研究采用丝裂霉素致半数死亡的黑色素瘤传代细胞株Ｂ１６为肿瘤特异性抗原，与Ｃ５７ＢＬ／６Ｊ小鼠脾淋巴细胞在ｒＩＬ－２的共同作用下。结果诱导出的Ｂ１６细胞特异杀伤Ｔ细胞，对Ｂ１６细胞在形态上和功能上有明显的杀伤作用。Objective To study the killing ability of tumor specific cytotoxic T cells on target tumor cells.Method The authors use half deathal melonoma cell line B16 cell treated with MMC as tumor specific antigen, and C57BL/6J mice spleenic lymphocytes were co cultured with it under the co existing of rIL 2.Result The result has showed that B16 tumor specific killing T cell were induced out. The cytotoxic T cells have dramatically killing effects on B16 cells morphology and function.Conclusion The results suggested that tumour specific cytotoxic T cells can be acquired using the method as in the study.

关 键 词：肿瘤特异性抗原 细胞毒T细胞 B16细胞 杀伤作用 

分 类 号：R730.3[医药卫生—肿瘤]