血清脂蛋白a和载脂蛋白a(TTTTA)(n)基因多态性与血管性认知功能障碍的关系  

Relation of pentanucleotide repeat polymorphism of apolipoprotein(a) gene to plasma lipoprotein(a) level among chinese patients with vascular cognitive impairment

作　　者：于爱玲[1,2] 赵仁亮[1] 王勤[2] 秦丽晨[2] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003 [2]泰山医学院附属泰安医院,山东泰安271000

出　　处：《泰山医学院学报》2010年第6期405-408,共4页Journal of Taishan Medical College

摘　　要：目的探讨载脂蛋白a(TTTTA)n基因多态性在血管性认知功能障碍患者中的分布状况及其与血清脂蛋白a[1 ipoprote in(a),Lp(a)]水平的关系。方法2008年1月至2009年8月在我院诊治的血管性认知功能障碍患者122例,健康对照组88例。运用免疫投射比浊法测定血清Lp(a)浓度,同时用聚合酶链反应(PCR)和聚丙烯酰胺凝胶电泳法测定载脂蛋白a[apolipoprote ina,Apo(a)]基因的5’调控区(TTTTA)n基因的多态性,并加以分析。结果共检出8种等位基因,16种基因型,常见的基因型为8/8、8/9、5/8、9/9四种。两组中5/8基因型携带者的血清Lp(a)水平均高于其他基因型携带者(P<0.05);高Lp(a)组5/8基因型携带者多于低Lp(a)组(P<0.05);且高Lp(a)组中血管性认知功能障碍患者所占比例明显高于低Lp(a)组(P<0.05)。结论血清Lp(a)是血管性认知功能障碍患者的高危因素。Apo(a)(TTTTA)(n)5/8基因型携带者血清Lp(a)水平升高,Apo(a)(TTTTA)n基因多态性可能与血管性认知功能障碍发病相关。Objective： Investigate the distribution of 5＇pentanucleotide TTTTA repeat polymorphism of apolipoprotein（a）[Apo（a）]gene among Chinese patients with vascular cognitive impairment（VCI）,and its relationship with blood serum lipoprotein（a）level.Methods： The examined objects were composed of two groups：122 patients with VCI and 88 healthy controls.DNA was extracted from the peripheral blood of 122 patients with VCI and 88 healthy controls.Blood serum Lp（a）level was measured by immunoturbidimetry.Pentanucleotide TTTTA repeat polymorphism of Apo（a）gene was determined by polymerase chain reaction（PCR）and subsequent polyacrylamide gel electrophoresis.Then we can contrast analysis.Results： Eight alleles and 16 genotypes in Apo（a）pentanucleotide TTTTA repeat were detected.The frequent genotype was 8/8、8/9、5/8 and 9/9.In the two controls blood serum Lp（a）levelof 5/8 genotype carrier was higher than the other genotype carrier＇s.The proportion of VCI patients in high blood serum Lp（a）level object was obviously higher than the low blood serum Lp（a）level object.Conclusion： Blood serum Lp（a）is an risk factor for VCI patient.The blood serum LP（a） level in 5/8 genotype carrier setted up.Pentanucleotide TTTTA repeat polymorphism of Apo（a）gene maybe associate with the invasion of VCI.

关 键 词：脂蛋白A 载脂蛋白A 基因多态性 血管性认知功能障碍 

分 类 号：R749.13[医药卫生—神经病学与精神病学]