乙酰肝素酶在非黑素性皮肤癌中的表达及临床意义  

The expression of heparanase in non-melanoma skin cancer and its clinical significance

作　　者：何荣国[1] 邹东奇[2] 田华[1] 武钦学[1] 邬运学[1] 

机构地区：[1]广州市第十二人民医院皮肤科,广州510620 [2]广东省中山大学肿瘤防治中心

出　　处：《中国医师杂志》2010年第8期1041-1044,共4页Journal of Chinese Physician

摘　　要：目的 探讨乙酰肝素酶（heparanase,Hpa）在非黑素性皮肤癌[基底细胞癌（BCC）和皮肤鳞状细胞癌（CSCC）]组织中的表达及其与CSCC病理分级和淋巴结转移的关系.方法 采用RT-PCR法和免疫组化法检测38例CSCC、33例BCC及19例正常皮肤组织中Hpa的表达水平,分析Hpa的表达与CSCC临床病理特征的关系.结果 CSCC组织中Hpa mRNA、Hpa蛋白的阳性表达率（68.4%、60.5%）均显著高于它们在BCC组织（24.2%、18.2%）和正常皮肤组织（10.5%、5.3%）中的表达（均P〈0.01）;CSCC组织中有淋巴结转移（92.3%、84.6%）和Broders分类Ⅲ～Ⅳ级Hpa mRNA、Hpa蛋白的阳性表达率（92.3%、84.6%）明显高于无淋巴结转移（56.0%、48.0%）和Ⅰ、Ⅱ级（56.0%、48.0%）中的表达（均P〈0.05）.结论 CSCC组织中Hpa表达高于BCC组织和正常皮肤组织,并与CSCC病理分级和淋巴结转移有关.Objective To investigate the expression of heparanase in non-melanoma skin cancer including cutaneous squamous cell carcinoma （CSCC） and basal cell carcinoma （BCC） and the relationship among heparanase expression and pathologic grading and lymph nodes metastasis of CSCC. Methods The expressions of heparanase were detected by RT-PCR assay and immunohistochemical staining in 38 cases of CSCC, 33 BCC and 19 normal human skin tissues. Then the relationship between heparanase expression and CSCC clinical pathological features was explored. Results The positive rate （ 68.4%, 60. 5% ） of heparanase mRNA and heparanase in CSCC （24. 2%, 18. 2% ） was significantly higher than those of BCC and normal skin tissue （ 10. 5% ,5.3% ） （ P 〈0. 01 ） . Heparanase expression rate in CSCC with lymph nodes metastasis （92. 3% ,84.6% ） and Ⅲ～Ⅳ（Broders） （92. 3% ,84. 6% ） was higher than those in tumor without lymph nodes metastasis （56. 0% ,48.0% ） and Ⅰ～Ⅱ （Broders） （56. 0% ,48. 0% ） （ P 〈0. 05）. Conclusions Heparanase was over-expressed in CSCC compared with BCC and normal skin tissues. Expression of heparanase was correlated to pathologic grade and lymph nodes metastasis of CSCC.

关 键 词：葡糖醛酸糖苷酶/代谢 皮肤肿瘤/代谢 肿瘤 基底细胞/代谢 肿瘤 鳞状细胞/代谢 

分 类 号：R739.5[医药卫生—肿瘤]