NK/T细胞淋巴瘤细胞凋亡和增殖的特征及意义  

The study of significance and features of cell apoptosis and proliferation of NK/T cell lymphoma

作　　者：明萌[1] 王大斌[1] 桂卫星[1] 易建华[2] 邹典定[3] 

机构地区：[1]十堰郧阳医学院附属太和医院儿科,湖北442000 [2]郧阳医学院附属太和医院病理科,442000 [3]武汉大学中南医院儿科,430071

出　　处：《临床肿瘤学杂志》2010年第8期699-702,共4页Chinese Clinical Oncology

基　　金：湖北省教育厅重点科研项目(B200624014)

摘　　要：目的探讨NK/T细胞淋巴瘤细胞凋亡和增殖的特征及意义。方法应用TdT介导的dUTP缺口末端标记(TUNEL)技术和免疫组织化学链霉素抗生物素-过氧化酶连接法(SP法)检测25例NK/T细胞淋巴瘤组织和10例反应性增生淋巴组织中的细胞凋亡、增殖细胞核抗原(PCNA)表达水平。结果 25例NK/T细胞淋巴瘤组织中细胞凋亡指数(AI)平均为(1.92±0.86)%,细胞增殖指数(PI)平均为(41.48±5.10)%;10例反应性增生淋巴组织AI平均为(6.70±1.89)%,PI平均为(20.10±2.77)%;与反应性增生淋巴组织相比,NK/T细胞淋巴瘤组织中AI显著减少(t=10.80,P<0.01),PI显著增大(t=12.39,P<0.01)。NK/T细胞淋巴瘤组织中AI与PI呈显著性正相关(r=0.69,P<0.01)。结论 NK/T细胞淋巴瘤组织中细胞凋亡减少,细胞增殖活跃,细胞凋亡与增殖的失衡在NK/T细胞淋巴瘤的发生发展中可能起重要作用。Objective To study features of cell apoptosis and proliferation and its clinical significance of NK/T cell lymphoma. Methods TdT-mediated dUTP nick end labeling （TUNEL） and immunohistochemistry were used to study cell apoptosis and expression of proliferation cell nuclear antigen （PCNA） in 25 NK/T cell lymphoma and 10 reactive lymphoid tissues. Results Apoptotic index （AI） and proliferative index （PI） averaged （ 1.92 ± 0. 86 ） % and （41.48 ±5.10） % respectively in the 25 NK/T cell lymphomas,and （6. 70 ±1.89） % and （20. 10 ±2. 77） % in the 10 reactive lymphoid tissues. Compared with reactive lymphoid tissues, AI was significantly reduced in NK/T cell lymphoma （ t = 10. 80, P 〈 0. 01 ） while PI significantly increased （ t = 12. 39, P 〈 0. 01 ）. In addition, in NK/T cell lymphoma, AI and PI were positively related （ r = 0. 69, P 〈 0. 01 ）. Conclusion In NK/T cell lymphoma, cell apoptosis reduce while cell proliferation is incneosing. The imbalance between cell apoptosis and cell proliferation is closely related to the development and progression of NK/T cell lymphoma.

关 键 词：NK/T细胞淋巴瘤 凋亡 增殖细胞核抗原 

分 类 号：R733.1[医药卫生—肿瘤]