间充质干细胞移植后在溃疡性结肠炎大鼠体内的迁移  

Endosomatic transference of transplanted bone marrow mesenchymal stem cells in rat of ulcerative colitis

作　　者：段征[1] 徐艳华[1] 陈小云[1] 汪维伟[1] 

机构地区：[1]重庆医科大学基础医学院组织胚胎学教研室、干细胞与组织工程研究室,重庆400016

出　　处：《重庆医科大学学报》2010年第8期1152-1155,共4页Journal of Chongqing Medical University

摘　　要：目的:观察大鼠骨髓间充质干细胞(Bone marrow mesenc hymal stem cells,BMSCs)静脉移植后在体内的迁移。方法:SD大鼠分为A组[免疫结合TNBS/ethanol诱导溃疡性结肠炎(Ulcerative colitis,UC)模型]和B组(正常大鼠),均尾静脉注射1ml荧光标记的BMSCs。移植后第3、7、14d各处死5只,取材做恒冷切片,荧光显微镜下观察BMSCs在骨髓、肺、肝、脾以及消化道内分布,测定荧光的积分光密度(Integral optical density,IOD)值。结果:BMSCs移植后多分布于血管丰富器官,7d时肺内荧光IOD值最高,14d时骨髓内IOD值最高,但无统计学意义。14d时,A组结肠的IOD值高于同组其余消化道各段以及B组结肠(P<0.05)。结论:静脉移植的BMSCs早期主要分布在血供丰富的器官中,迁移至受损结肠的BMSCs高于正常结肠。Objective：To investigate the transference of transplanted bone marrow mesenchymal stem cells （BMSCs）in rat of ulcerative colitis. Methods：SD rats were randomly distributed into group A colitis induced by immune-combined TNBS/ethanol and group B as control. All rats received caudal vein injection with 1 ml of fluorescence marked BMSCs,then 5 rats were sacrificed at day 3,7 and 14 respectively in each group. Cryostat sections of bone marrow,lung,liver,spleen and gut were made to observe the distribution of BMSCs in these organs,and the fluorimetric integral optical density（IOD）were analyzed. Results：BMSCs were mainly distributed in organs which richs vessels. The highest IOD level of BMSCs in lung was on the 7th day,and was on the 14th day in bone marrow. On the 14th day,the IOD level of BMSCs in colon of group A were higher than those of other segments of digestive tube and also higher than those in group B （P0.05）. Conclusion：Transplanted BMSCs in earlierperiod were mainly distributed in the organs with rich blood supply,and BMSCs migrated to the injured colon are higherthan those of normal colon.

关 键 词：骨髓间充质干细胞 移植 溃疡性结肠炎 大鼠 

分 类 号：R574.1[医药卫生—消化系统] R-332[医药卫生—内科学]