MMP7反义寡核苷酸对肺腺癌A549细胞粘附和侵袭能力的影响  

Effects of MMP7 antisense oligodeoxynucleotide on adhesion and invasion of human lung adenocarcinoma cell A549

作　　者：杨波[1] 唐伏秋[2] 高建飞[1] 饶智国[1] 王志新[3] 

机构地区：[1]广州军区武汉总医院肿瘤科,武汉430070 [2]广州军区武汉总医院干部病房二科,武汉430070 [3]第三军医大学新桥医院全军肿瘤研究所,重庆400037

出　　处：《重庆医科大学学报》2010年第8期1160-1163,共4页Journal of Chongqing Medical University

摘　　要：目的:观察基质溶解素(Matrilysin,MMP7)反义寡核苷酸(Antisense oligodeoxynucleotide)对肺腺癌A549细胞体外粘附和侵袭能力的影响。方法:采用硫代磷酸修饰的MMP7 ASODN,通过脂质体导入A549细胞,RT-PCR检测MMP7 mRNA表达;平板粘附模型和Boyden chamber模型比较A549细胞的粘附和侵袭能力。结果:MMP7 ASODN转染A549细胞后,与未转染组和脂质体组比较,RT-PCR电泳条带相对光密度值显著降低(P<0.01)。粘附于平板和穿过Boyden chamber的细胞数有明显下降(P<0.01)。结论:MMP7 ASODN可以有效抑制肺腺癌A549细胞株MMP7基因的表达,降低细胞粘附和侵袭能力。Objective：Toinvestigate the influence of MMP7 antisense oligodeoxynucleotide（ASODN）on adhesion and invasion ofhuman lung adenocarcinoma cell line A549.Methods：Phosphorothioate MMP7 ASODN was transfected to A549 cells mediated by liposome. The expression of MMP7 was examined by RT-PCR.The adhesive and invasive ability were examined by plate adhesion model and Boyden Chamber transwell assay.Results：After MMP7 ASODN was transfected,the relative optical density（ROD）of electrophoresis strip was decreased obviously（P0.01）.Compared with A549 group and liposome group,the cells adhering to plate and permeating septum of Boyden chamber were decreased markedly（P0.01）.Conclusion：The MMP7 ASODN could effectively inhibit the expression of MMP7 and reduce the adhesive and invasive ability of A549.

关 键 词：基质溶解素 反义寡核苷酸 肺腺癌A549细胞 粘附 侵袭 

分 类 号：R342.2[医药卫生—基础医学]