谷氨酰胺体外对大鼠晶状体上皮细胞HSP70和NF-κB表达的影响  

Influence of glutamine on the expression of HSP70 and NF-κB

作　　者：刘瑜[1] 鲁建华[1] 张文芳[1] 李姗姗[1] 曲来强[1] 

机构地区：[1]兰州大学第二医院眼科,中国甘肃省兰州市730030

出　　处：《国际眼科杂志》2010年第9期1662-1664,共3页International Eye Science

摘　　要：目的:探讨谷氨酰胺(Gln)体外对晶状体上皮细胞HSP70和NF-κB的作用。方法:采用ELISA法检测在MEM培养液里培养3,12和24h晶状体上皮细胞HSP70和NF-κB的表达水平。结果:谷氨酰胺注射各组HSP70蛋白的表达量均显著高于对照组(P<0.05),谷氨酰胺注射各组NF-κB的表达较对照组显著降低(P<0.05)。结论:谷氨酰胺能诱导晶状体上皮HSP70蛋白的表达,抑制NF-κB的表达。AIM： To study the effect of glutamine（Gln） on the expression of heat shock protein 70（HSP70） and NF-κB in vitro. METHODS：HSP70 and NF-κB expression was detected by ELISA in different times（3,12,24 hours）after the lenses of rats were cultivated in MEM cultural medium. RESULTS： The expression of HSP70 in the Gln group was significantly higher than that of the control group（P〈0.05;On the contrary, the expression of NF-κB in the Gln group was significantly lower than that of control group（P〈0.05）. CONCLUSION： Gln can induce the expression of HSP70 in the lens epithelial cells of rat and inhibit the expression of NF-κB.

关 键 词：谷氨酰胺 热休克蛋白70 核转录因子 晶状体上皮细胞 紫外线 

分 类 号：R776.1[医药卫生—眼科]