GM6001对宫颈鳞癌HCE1多细胞球体浸润人脐静脉内皮细胞的影响  

Effect of GM6001 on the model that cervical squamous carcinoma cell line HCE1 multicellular spheroids invade live human umbilical vein endothelium cell monolayers

作　　者：兰菁[1] 吴宜林[1] 

机构地区：[1]中南大学湘雅二医院妇产科,长沙410011

出　　处：《中南大学学报（医学版）》2010年第8期868-874,共7页Journal of Central South University ：Medical Science

基　　金：湖南省教育厅科研基金(2050205)~~

摘　　要：目的:探讨基质金属蛋白酶(MMP)-9在宫颈鳞癌HCE1多细胞球体中的表达与宫颈癌浸润的关系,研究MMP抑制剂GM6001对HCE1多细胞球体浸润人脐静脉内皮细胞(HUVECs)的影响。方法:建立宫颈鳞癌HCE1多细胞球体浸润HUVECs模型;采用免疫组织化学法检测宫颈鳞癌HCE1单层细胞、多细胞球体MMP-9的表达;光镜下观察GM6001(2.5,12.5μmol/L)干预宫颈鳞癌HCE1多细胞球体浸润模型的形态学改变并检测干预后HCE1浸润模型中MMP-9的表达。结果:成功建立HCE1浸润模型;HCE1多细胞球体中MMP-9的阳性表达率明显高于HCE1单层细胞(P<0.05);与对照组比较,GM6001低剂量组和高剂量组HCE1多细胞球体浸润能力抑制率分别为26.09%和92.95%(P<0.05),其浓度增加抑制能力增强;宫颈鳞癌HCE1多细胞球体浸润模型(第4天)GM6001高剂量组HCE1多细胞球体中MMP-9表达较对照组明显下调(χ2=7.033,P<0.05)。结论:HCE1多细胞球体的浸润能力增强,可能与多细胞球体中MMP-9表达上调有关;GM6001可以下调HCE1多细胞球体中MMP-9的表达,部分阻断HCE1多细胞球体对单层HUVECs的浸润作用。Objective To investigate the relationship between matrix matrix metalloproteinases（MMP）-9 expression in HCE1/multicellular spheroids（MCS） and invasion of cervical carcinoma,and to explore the effect of MMPs inhibitor GM6001 on the model that HCE1/MCS invade live human umbilical vein endothelium cell（HUVEC）.Methods We established the invasion model by coculturing HCE1/MCS and HUVEC,and detected the MMP-9 expression in HCE1 monolayer cells（HCE1/MC）,HCE1/MCS and HUVEC using immunohistochemistry and treated the HCE1/MCS invasion model with GM6001.Results A cervical squamous carcinoma cell HCE1 infiltrating model was established.Compared with that in HCE1/MC,MMP-9 expression in the HCE1/MCS was significantly higher（P0.05）.Compared with the control group,the invasion of HCE1/MCS was inhibited by 26.09 % and 92.95%（P0.05） in the GM6001 2.5 μmol/L and 12.5 μmol/L group.MMP-9 expression in HCE1/MCS in the GM6001 12.5 μmol/L group was obviously lower than that in the control group（P0.05）.With the increase of the concentration of GM6001,the inhibition increased.Conclusion The enhancement of HCE1/MCS invasion may be related to the up-regulation of MMP-9 expression in HCE1/MCS.GM6001 can down regulate the MMP-9 expression in HCE1/MCS and partially block the HCE1/MCS invasion to HUVEC.

关 键 词：宫颈癌 多细胞球体 金属蛋白酶-9 金属蛋白酶抑制剂 

分 类 号：R737.33[医药卫生—肿瘤]