水稻阶段性返白突变体的鉴定和候选基因分析  

Identification and candidate gene analysis of stage green-revertible albino mutant in rice(Oryza sativa L.)

作　　者：张向前[1] 李晓燕[2] 朱海涛[1] 王涛[1] 解新明[1] 

机构地区：[1]华南农业大学农学院,广州510642 [2]西北农林科技大学农学院,杨凌712100

出　　处：《科学通报》2010年第23期2296-2301,共6页Chinese Science Bulletin

基　　金：国家自然科学基金(30900884);广东省自然科学基金(9451064201003804);高等学校博士学科点专项科研基金(20094404120011)资助项目

摘　　要：从粳稻品种中花11的后代中发现了一个叶色突变体sgra,该突变体幼苗期叶色正常,而6~8叶期以后新生叶白化,随后白化叶转绿.突变体的遗传分析表明,该突变体表型受1对隐性核基因控制.利用籼粳杂交F2群体对突变位点进行了基因定位,将其定位于水稻第11染色体的2个标记ID343-11和PSM415之间,遗传距离分别为0.9和1.0cM.随后,利用已公布的水稻序列和SSR标记,在两标记间发展了9对新的标记,进一步将SGRA基因定位在IDM-2和RM26739之间,物理距离约为17.6kb.对野生型和突变体候选区段基因组DNA测序分析表明,候选基因编码一个ABC转运蛋白.A rice leaf color mutant was obtained from a japonica variety,Zhonghua 11.The mutant and Zhonghua 11 had very few differences in seedling leaf color at the 1- to 5-leaf stage.The mutant had albino leaves at the 6- to 8- leaf stage and then reverted to the normal green color.Genetic analysis showed that the mutant was controlled by a single recessive gene,designated as sgra（stage green-revertible albino）.A genetic linkage map was constructed using a large F2 mapping population derived from a cross between the mutant and an indica variety,Huajinxian 74.The SGRA gene was mapped between the markers ID343-11 and PSM415.Fine mapping,conducted with nine newly developed molecular markers based on the sequence diversity around the region harboring SGRA in Nipponbare and 93-11,delimited SGRA to a 17.6-kb region between markers IDM-2 and RM26739.Genomic sequencing of the region between these two markers in Zhonghua 11 and the sgra mutant revealed that the candidate SGRA gene encodes an ABC transporter.

关 键 词：水稻 叶色突变体 ABC转运蛋白 INDEL标记 

分 类 号：S511[农业科学—作物学]