砂梨EST-SSR引物开发及其应用  

Development and Application of EST-SSRs in Sand Pear

作　　者：崔海荣[1] 刘金义[1] 佟兆国[1] 王新卫[1] 章镇[1,2] 乔玉山[1,2] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]教育部园艺作物种质创新与利用工程研究中心,南京210095

出　　处：《西北植物学报》2010年第8期1551-1556,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家高技术研究与发展计划重点项目(2006AA100108)

摘　　要：利用GenBank和GDR数据库中的995条梨EST序列开发砂梨SSR引物,并根据开发的EST-SSR引物对砂梨西子绿×喜水F1群体的遗传变异进行分析.结果发现：（1）60个SSR位点分布于54条EST序列中,占整个EST数据库的5.4%,其中二核苷酸重复基元出现频率最高,达51.7%,其次为三核苷酸重复基元占25%.23类重复基序中AT重复基序出现的频率最高,为32.3%.（2）利用开发的25对EST-SSR引物对西子绿×喜水F1群体的遗传变异分析结果表明,其中9对引物呈现多态性,多态性引物占设计引物的36%;多态性引物扩增产物在F1群体中的等位基因数（No）平均为2,有效等位基因数（Ne）平均为1.932 4,平均杂合度观测值（Ho）和期望杂合度（He）分别为1和0.480 9.EST sequences in GenBank and GDR database were used to develop from pear（Pyrus L.） SSR markers.25 pairs of EST-SSR primers were developed for analysing genetic diversity within F1 progeny of Pyrus pyrifolia Nakai ＇Xizilü＇×＇Kisui＇.The result showed that 60 SSR sequences were selected in the 54 EST sequences and accounted for 5.4% of total ESTs.EST sequences were found to contain repeat elements,in which dinucleotide repeats,accounting for 51.7%（31） of EST-SSRs were dominant,then trinucleotide repeats,accounting for 25%（15） of EST-SSR.In 23 motif types,AT（32.3%） dinucleotide repeats had the highest occurrence frequency.9 of 25 EST-SSR primer pair amplification results showed polymorphism,with the ratio of 36% in the designed SSR primers.According to the amplification bands by the 9 primer pairs,the average number of alleles（No） and the effective number of alleles（Ne）,were 2 and 1.932 4,and the mean observed heterozygosity（Ho） and the mean expected heterozygosity（He） were 1 and 0.480 9,respectively.

关 键 词：砂梨 EST-SSR 分子标记 

分 类 号：Q346.5[生物学—遗传学]