外源亚精胺对Ca（NO3）2胁迫下黄瓜幼苗生长和活性氧代谢的影响  

Effects of Exogenous Spermidine on Growth and Active Oxygen Metabolism in Cucumber Seedlings under Ca（NO3）2 Stress

作　　者：金春燕[1] 孙锦[1] 郭世荣[1] 

机构地区：[1]南京农业大学园艺学院农业部南方蔬菜遗传改良重点开放实验室

出　　处：《西北植物学报》2010年第8期1627-1633,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家重点基础研究发展计划(2009CB119000);现代农业产业技术体系建设专项资金资助;国家科技支撑计划项目(2008BADA6B07);江苏省农业三项工程项目[SX(2008)026]

摘　　要：以温室专用黄瓜品种津优3号幼苗为材料,采用营养液栽培方法,研究了叶面喷施1 mmol·L^-1亚精胺（Spd）对60 mmol·L^-1硝酸钙胁迫下黄瓜幼苗生长和植株体内活性氧代谢的影响.结果显示,Ca（NO3）2胁迫下,黄瓜幼苗叶片和根系O2·^-产生速率显著增加,超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）等抗氧化酶活性升高,同时MDA含量和相对电导率明显提高,显著降低了黄瓜幼苗的株高、鲜重和干重;外源喷施Spd提高了硝酸钙胁迫下黄瓜幼苗叶片和根系SOD、POD和CAT活性,降低了O2·^-产生速率,MDA含量及相对电导率显著下降.由此可见,外源Spd可通过提高黄瓜幼苗SOD、POD和CAT等保护酶活性来增强其对体内活性氧的有效清除能力,降低膜质过氧化伤害程度,从而缓解硝酸钙胁迫对黄瓜幼苗生长的抑制.With special cucumber varieties for greenhouse ＇Jinyou 3＇ as materials,effects of exogenous spermidine（Spd,1 mmol·L^-1） on seedling growth and active oxygen metabolism in cucumber seedlings under 60 mmol·L^-1 Ca（NO3）2 stress were investigated by hydroponic culture.The results showed that under Ca（NO3）2 stress,the O2·^- producing rate increased,the activities of SOD,POD and CAT were significantly higher than those of control.The content of MDA and relative electric conductivity were significantly increased,while the seedlings height,the fresh and dry weight were significantly decreased.Exogenous spermidine application on the leaf further increased the activities of antioxidant enzyme,increased scavenging of reactive oxygen,which reduced the content of MDA,the O2·^- producing rate and relative electric conductivity were decreased.These results show that exogenous Spd can effectively remove the reactive oxygen species and reduces the damage from membrane lipid oxidative,by which mitigate the harmful effects from Ca（NO3）2 stress on plants,and promote the growth of cucumber.

关 键 词：硝酸钙胁迫 黄瓜 亚精胺 活性氧代谢 抗氧化酶 

分 类 号：Q945.78[生物学—植物学]