地山雀线粒体基因组全序列的测定和分析  

Sequencing and Analysis of the Complete Mitochondrial Genome of Pseudopodoces humilis(Aves,Paridae)

作　　者：杨超[1] 雷富民[2] 黄原[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062 [2]中国科学院动物研究所动物进化与系统学重点实验室,北京100101

出　　处：《Zoological Research》2010年第4期333-344,共12页动物学研究（英文）

基　　金：国家杰出青年科学基金(30925008);自然基金资助项目(30870270);中国科学院动物进化与系统学重点实验室开放课题资助项目(O529YX5105)

摘　　要：基于长距PCR扩增及保守引物步移法测定并注释了地山雀(Pseudopodoces humilis)的线粒体基因组全序列。结果表明,地山雀线粒体基因组全长1.6809万bp,A+T含量为52.9%,37个基因排列顺序与红原鸡一致。蛋白质基因的起始密码子中,除COI基因为GTG外,其余均为ATG。ND1和ND5基因终止密码子为AGA;COⅡ基因为AGG;COⅢ和ND4基因为不完全终止密码子T;其余基因均为典型的TAA或TAG。预测了22个tRNA 基因的二级结构,发现tRNA Ser(AGN)缺少DHU臂, tRNA Phe的TψC臂存在一单核苷酸插入。预测的地山雀12S和16SrRNA二级结构分别包括3个结构域47个茎环和6个结构域60个茎环。控制区位于 tRNA Glu和 tRNA Phe之间,长度1240bp。控制区结构为高变I区、中央保守Ⅱ区和保守序列Ⅲ区3个结构域。The complete sequence of Pseudopodoces humilis mitochondrial genome was determined by using long PCR and conserved primers walking approaches.The results showed that the entire mitochondrial genome of Pseudopodoces humilis was 16 809 bp in length with 52.9% A＋T content,and the 37 genes had the same gene order with that of Gallus gallus.All protein coding genes of the Pseudopodoces humilis mitochondrial genome started with ATG codon,except for COI with GTG.For terminate codon usage,most of genes terminate with codons TAA or TAG,ND1 and ND5 were AGA,COII was AGG,and the COIII and ND4 genes had an incomplete termination codon（T）.The secondary structures of 22 tRNA were predicted,all tRNA can form typical secondary structure,except tRNASer（AGN） with an absence of DHU arm,and tRNAPhe with an extra nucleotide inserted in the TψC arm.The predicted secondary structures of 12S rRNA and 16S rRNA exhibit 47 helics in 3 structural domains and 60 helics in 6 domains,respectively.The control region of Pseudopodoces humilis located between tRNAGlu and tRNAPhe with 1 240 bp in length,and has structure domains found in that of the other birds.

关 键 词：地山雀 雀形目 山雀科 地山雀属 线粒体全基因组 RNA二级结构 中央保守区 

分 类 号：Q953[生物学—动物学]