缺氧诱导因子-1α与P糖蛋白在喉鳞癌中的表达及临床意义  

Expression and significance of hypoxia-inducible factor 1α and p-glycoprotein in laryngeal squamous cell carcinoma

作　　者：李大伟[1] 高尚[1] 陈歆维[1] 王果[1] 董频[1] 

机构地区：[1]上海交通大学附属第一人民医院耳鼻喉头颈外科,上海200080

出　　处：《山东大学耳鼻喉眼学报》2010年第4期6-9,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：上海市生物医药领域重点科技项目(09411951000);上海市级医院临床科研资源共享平台(SHDC12007206)

摘　　要：目的探讨H IF-1α、P-gp在喉鳞癌及喉黏膜正常上皮中的表达差异,两者在喉鳞癌中表达与临床病理特征的关系及表达间的相关性。方法应用免疫组化染色技术检测H IF-1α和P-gp在86例喉鳞癌及32例喉黏膜正常上皮中的表达。结果H IF-1α与P-gp在喉鳞癌组织中阳性表达率均高于喉黏膜正常上皮组织(P<0.05)。喉鳞癌组织中H IF-1α阳性表达率与肿瘤临床分期、淋巴结转移有关(P<0.05);P-gp阳性表达率与肿瘤临床分期、组织学分级、淋巴结转移有关(P<0.05)。H IF-1α和P-gp蛋白在表达水平上成正相关(r=0.442,P<0.01)。结论H IF-1α与P-gp的高表达可能在喉鳞癌病情进展中起一定作用,并可作为评估喉鳞癌临床病理特征的有效生物学指标。Objective To investigate expressions of HIF-1α （ Hypoxia-inducible factor 1α） and P-gp （P-glycoprotein） in human laryngeal squamous cell carcinoma（LSCC） and normal laryngeal mucosa, and to analyze their relationship with clinicopathological features. Methods Expression of HIF-1α and P-gp of 86 cases of LSCC and that of 42 cases of normal laryngeal mucosa were detected by immunohistochemistry. Results The positive rates of HIF-1α and P-gp proteins were significantly higher in LSCC than those in normal tissues （P 〈0.05）. Expression of HIF-1α protein was correlated with the TNM stage and lymph node metastasis （ P 〈 0.05 ）, but not histological differentiation and primary site（ P 〉0.05 ）. The level of P-gp expression was also associated with the TNM stage, lymph node metastasis, histological differentiation of LSCC but not primary site （ P 〉 0.05 ）. There was a positive correlation between the expressions of HIF-1α and P-gp proteins （ r = 0. 442, P 〈 0.01 ）. Conclusion High expressions of HIF-1α and P-gp proteins in LSCC may play important roles in the progression of the disease. HIF-1α and P-gp may be two potential biomarkers in the assessment of clinicopathological features of LSCC.

关 键 词：喉肿瘤 癌 鳞状细胞 缺氧诱导因子-1 P糖蛋白 

分 类 号：R739.65[医药卫生—肿瘤]