Bisindolylmaleimide Ⅷ对Jurkat T细胞生物学行为的影响  

Effects of bisindolylmaleimide Ⅷ on the biologic behavior of Jurkat T cells

作　　者：吕梦捷[1] 曾耀英[1] 赵晓慧[1] 宋兵[1] 

机构地区：[1]暨南大学组织移植与免疫中心,广州510632

出　　处：《免疫学杂志》2010年第8期649-652,668,共5页Immunological Journal

基　　金："973"国家重大基础研究项目(2006CB504200和2004CB-720100);广东省基金项目(2006B36030016);广州市科技局科技攻关重点项目(2006Z-E0091);生物化学与分子生物学广东省重点学科资助

摘　　要：目的研究BisⅧ对Jurkat T细胞活化、增殖、周期和凋亡的影响,并对其抗肿瘤机制进行初步探讨。方法以Jurkat T细胞为模型;以双色荧光抗体染色技术结合流式细胞术检测BisⅧ对T淋巴细胞早期活化抗原CD69的表达的影响;利用噻唑蓝(MTT)比色法观察BisⅧ对JurkatT细胞增殖影响;使用倒置显微镜观察BisⅧ对Jurkat T细胞集落形成的情况的影响;采用碘化丙锭(PI)染色检测BisⅧ对Jurkat T细胞周期的影响;应用钙黄绿素(Calcien-AM)染色检测BisⅧ对JurkatT细胞凋亡的影响。结果终浓度为5、10、20μmol/L的BisⅧ对Jurkat T细胞的早期活化抗原CD69的表达具有明显的抑制作用(P<0.05);BisⅧ对Jurkat T细胞增殖具有显著的抑制作用(P<0.01),且呈剂量依赖性。随着BisⅧ浓度的增高,Jurkat T细胞停滞于G0/G1期;BisⅧ对JurkatT细胞凋亡具有明显的促进作用,并呈剂量依赖性。结论 BisⅧ对JurkatT细胞细胞生物学行为有抑制作用,是潜在治疗白血病的抗肿瘤药物。We aim to investigate the effects of bisindolylmaleimide（Bis） Ⅷ on the activation,proliferation,cell-cycle and apoptosis of Jurkat T cells,as well as to elucidate the mechanism of the process.Jurkat cells were divided into blank control group and Bis Ⅷ-treated groups.The expression level of CD69 on the surface of Jurkat T cells was evaluated by the technique of fluorescinconjugated monoclonal antibody double labeling;MTT method was used to analyze the proliferation of Jurkat T cells;the growing situation of Jurkat T cells was observed using microscope;PI staining and flow cytometry were used to analyzed the cell cycle;the apoptosis of Jurkat T cells was measured by Calcein staining.The experiment result showed that the expressions of CD69 of Jurkat T cells treated with different concentrations （5 μmol/L,10 μmol/L,20 μmol/L） of Bis Ⅷ decreased （P〈0.05）,and Bis Ⅷ could significantly inhibited the proliferation of Jurkat T cells in a dose-dependent manner （P〈0.01）.With the growing of the concentration of Bis Ⅷ,the cell cycle of Jurkat T cells was arrested in G0 /G1 phase.Furthermore,the Bis Ⅷ promoted the apoptosis of the Jurkat T cell in a dose-dependent manner.Thus,Bis Ⅷ can effectively inhibit the biologic behavior of Jurkat T cells,so we can conclude that Bis Ⅷ has potential to be a medicine of acute leukemia.

关 键 词：BisⅧ JurkatT细胞 活化 增殖 凋亡 

分 类 号：R730.2[医药卫生—肿瘤]