Gas、SS及SP在海洛因依赖大鼠空肠中的定位和表达  

Localization and expression of Gas, SS and SP in the jejunum of heroin-dependent rats

作　　者：刘霞[1] 梁文妹[1] 

机构地区：[1]贵阳医学院组织胚胎学教研室,贵州省贵阳市550004

出　　处：《世界华人消化杂志》2010年第21期2192-2196,共5页World Chinese Journal of Digestology

基　　金：教育部"春晖计划"基金资助项目;No.Z2005-1-52020;贵州省科技攻关基金资助项目;No.黔科合S字20071048~~

摘　　要：目的:探讨胃泌素(gastrin,Gas)、生长抑素(somatostatin,SS)和P物质(substanceP,SP)在海洛因依赖大鼠空肠的定位和表达.方法:成年SD大鼠66只,按配对原则随机分为正常对照组,盐水对照组和海洛因依赖组.按体质量逐日递增海洛因药液剂量,皮下注射.建立海洛因依赖动物模型.分别于10、17、24、31、38d取空肠组织.应用免疫组织化学SABC法及图像分析法观察空肠Gas-、SS-和SP-免疫反应(immunoreactive,IR)细胞的数量及IR强度变化.结果:与正常对照组,盐水对照组比较,海洛因依赖组Gas-、SS-和SP-IR细胞的数量(细胞数/视野)明显增加(P<0.05),其中Gas-、SS-IR细胞17d,SP-IR细胞24d增加最为显著(2.68±0.90vs1.84±0.47,1.84±0.62;2.56±0.65vs1.60±0.50,1.60±0.50;2.96±0.54vs1.76±0.44,1.80±0.50,均P<0.05).图像分析显示Gas-,SS-和SP-IR细胞的平均灰度值低于正常及盐水对照组(P<0.05).其中Gas-,SS-IR细胞24d最低,SP-IR细胞31d最低.结论:在海洛因依赖大鼠空肠中,Gas-、SS-和SP-IR细胞数量和表达量增加.AIM： To investigate the localization and expression of gastrin （Gas）, somatostatin （SS） and substance P （SP） in the jejunum of heroin-dependent rats.METHODS： Sixty-six adult Sprague-Dawley rats were randomly divided into three groups： nor-mal control group （NCG）, saline control group （SCG） and heroin dependence group （HDG）. A rat model of heroin dependence was established by subcutaneous injection of heroin. Jejunum tis-sue was excised on days 10, 17, 24, 31 and 38 af-ter heroin injection. Immunohistochemistry was performed to detect the expression and localiza-tion of Gas, SS and SP in jejunum tissue. RESULTS： Compared with the NCG and SCG groups, the numbers of Gas-, SS- and SP-immu-noreactive cells in the jejunum increased signif i-cantly in the HDG group （all P 0.05）, especially prominent on day 17 for Gas （2.68 ± 0.90 vs 1.84 ± 0.47 and 1.84 ± 0.62, P 0.05） and SS staining （2.56 ± 0.65 vs 1.60 ± 0.50 and 1.60 ± 0.50, P 0.05） and on day 24 for SP staining （2.96 ± 0.54 vs 1.76 ± 0.44 and 1.80 ± 0.50, both P 0.05）. The stain-ing intensity of Gas, SS and SP in the jejunum was signifi cantly lower in the HDG group than in the NCG and SCG groups （all P 0.05）.CONCLUSION： The numbers of Gas-, SS- and SP-IR cells signifi cantly increase in the jejunum of heroin-dependent rats.

关 键 词：胃泌素 生长抑素 P物质 空肠 海洛因依赖 

分 类 号：R749.64[医药卫生—神经病学与精神病学]