高效液相色谱法检测早期胃癌中胃液固有荧光的诊断价值  

Diagnostic value of gastric juice autofluorescence detected by high-performance liquid chromatography in early gastric cancer

作　　者：张月宁[1] 金新莲[1] 孟灵梅[1] 周丽雅[1] 林三仁[1] 耿秋明[2] 彭佳柔[3] 

机构地区：[1]北京大学第三医院消化科,北京市100083 [2]北京大学第三医院中心实验室,北京市100083 [3]北京大学医学部蛋白质组学国家重点实验室,北京市100083

出　　处：《世界华人消化杂志》2010年第21期2262-2265,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30371603;No.30672395~~

摘　　要：目的:研究高效液相色谱法检测早期胃癌中胃液固有荧光光谱的诊断价值.方法:选择2004-08/2008-03在北京大学第三医院胃镜检查患者的胃液,采用MonoQ离子交换高效液相色谱分析方法检测胃液固有荧光,比较早期胃癌与胃良性疾病间的荧光峰差异并进行统计分析.结果:胃液在MonoQ离子交换高效液相色谱条件下于保留时间30-32min之间规律出现一个强度最大的荧光峰,早期胃癌组均值明显高于胃良性病变组(P<0.001).以荧光强度0.12V为判别界值,诊断早期胃癌的敏感度是91.2%、特异度是87.8%.结论:高效液相色谱法检测胃液固有荧光用于诊断早期胃癌具有较高的敏感性和特异性.AIM： To evaluate the diagnostic value of gastric juice autofluorescence detected by high-liquid performance chromatography in early gastric cancer. METHODS： Gastric juice was collected from patients with different gastric diseases undergoing gastroscopy at our hospital from August 2004 to March 2008. Autofluorescence of gastric juice was detected by highperformance liquid chromatography, and different fluorescence peaks were statistically analyzed. RESULTS： An obvious autofluorescence peak at a retention time of 30-32 min could be detected, which was signif icantly different between early gastric cancer and gastric benign diseases （P 0.001）. At a critical fluorescence intensity value of 0.12 V, the sensitivity and specif icity of detection of gastric juice autofluorescence by highperformance liquid chromatography for the diagnosis of early gastric carcinoma were 91.2% and 87.8%, respectively. CONCLUSION： Detection of gastric juice autofluorescence by highperformance liquid chromatography has a high sensitivity and specif icity in the diagnosis of early gastric cancer.

关 键 词：胃癌 胃液 固有荧光 高效液相色谱 

分 类 号：R735.2[医药卫生—肿瘤]