用Nested-PCR检测广西黄皮与九里香黄龙病病原  

Nested-PCR Detection of the pathogen of Citrus Huanglongbing on Clausena lansium and Murraya paniculata

作　　者：邓崇岭[1] 陈传武[1] 赵小龙[2] 付慧敏[1] 陈国平[1] 白先进[2] 娄兵海[1] 吴礽超[1] 

机构地区：[1]广西壮族自治区柑桔研究所,广西桂林541004 [2]广西壮族自治区农业厅,南宁530022

出　　处：《中国农学通报》2010年第17期273-276,共4页Chinese Agricultural Science Bulletin

基　　金：科技部国际科技合作项目"柑桔优势品种繁育及其高效栽培关键技术研究"(2008DFA30900);农业部公益性行业(农业)科研专项"柑桔模式化栽培与贮藏技术研究"(nyhyzx07-08);广西农业重点科技计划项目"柑桔黄龙病综合防治技术研究"(NK200827);广西自然科学基金项目"黄皮;九里香抗柑桔黄龙病机理研究"(桂科自0728038)

摘　　要：运用Nested-PCR技术对来自广西部分市、县的40个黄皮和九里香进行柑桔黄龙病(CitrusHuanglongbing,HLB)病原检测,结果表明:从部分黄皮和九里香样品抽提的DNA中,可扩增出一条特异性的400bp条带,说明广西种植的黄皮和九里香均可感染黄龙病;在柑桔黄龙病疫区,黄皮和九里香黄龙病感染率分别为35.0%和40.0%,黄龙病感染率已相当高。为了减少柑桔黄龙病的发生,有利于对柑桔黄龙病进行综合防控,不宜在柑桔种植区栽种黄皮和九里香,铲除已感染黄龙病的植株。Nested-PCR was used for the detection of the pathogen of citrus Huanglongbing （HLB） on 40 samples ofClausena lansium andMurraya paniculata from several cities of Guangxi. The result demonstrated that a specific 400 bp band could be amplified from part of the DNA extracted from these plant materials, respectively. It indicated that Clausena lansium and Murraya paniculata from Guangxi could be infected by HLB. In HLB-infected area, HLB infection rates ofClausena lansium andMurraya paniculata were 35.0% and 40.0% , respectively. For the purpose of reduction of HLB infection rates and integrated control of HLB, Clausena lansium andMurraya paniculata should not be planted in the citrus planting area, and plants infected by HLB should be removed as soon as possible.

关 键 词：黄皮 九里香 黄龙病 NESTED-PCR 

分 类 号：Q789[生物学—分子生物学]