向日葵菌核病根际拮抗菌的筛选与鉴定及其拮抗机理的初步研究  

Screening and Identification of Antagonistic Bacteria from Sunflower Rhizosphere and Preliminary Study on the Antagonistic Mechanism

作　　者：侯启会[1] 丁延芹[1] 杜秉海[1,2] 靳奉理[1,2] 路晓萌[1] 孙臻鑫[1] 周瑞军 姚良同[1] 

机构地区：[1]山东农业大学生命科学学院,泰安271018 [2]山东农业大学农业微生物重点实验室,泰安271018 [3]栖霞市农业局,烟台265300

出　　处：《生物技术通报》2010年第9期138-142,153,共6页Biotechnology Bulletin

基　　金：山东省优秀中青年科研奖励基金(2006BS06012)

摘　　要：利用梯度稀释和对峙试验的方法,从向日葵根际土壤筛选到18株对向日葵菌核病病原菌—核盘菌(Sclerotiniasclerotiorum)有拮抗效果的细菌,其中1株具有较强的拮抗效果,命名为XRK4。经过形态观察及16S rDNA序列分析,将XRK4鉴定为地衣芽孢杆菌(Bacillus licheniformis)。研究表明XRK4具有β-1,3-1,4-葡聚糖酶活性。设计引物以XRK4的基因组DNA为模板扩增得到长度为732 bp的β-1,3-1,4-葡聚糖酶的完整基因,其开放阅读框架编码243个氨基酸。XRK4可能因产生葡聚糖酶,降解病原菌细胞壁而具有拮抗活性。Eighteen antagonistic bacteria were screened from sunflower rhizosphere,which is resistant to Sclerotinia sclerotiorum.One of the isolated strains named as XRK4 was identified as Bacillus licheniformis based on the morphologic characteristics and phylogenetic analysis of 16S rDNA.The results showed that XRK4 has β-1,3-1,4-glucanase activity.The open reading frame of β-1,3-1,4-glucanase gene is 732 bp encoding 243 amino acids.XRK4 might have antagonistic activity based on its β-1,3-1,4-glucanase gene.

关 键 词：向日葵根际 拮抗细菌 核盘菌 Β-1 3-1 4-葡聚糖酶 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S435.655[农业科学—植物保护]