花生壳总黄酮提取及其对自由基清除作用的研究  

Studies on Total Flavonoids Extracted from Peanut Hull and Its Hydroxyl Radical Scavenging Effects

作　　者：陈孝刚[1] 王志良[2] 王彧[2] 陈婷[2] 胡志军[1] 陈建秋[1] 

机构地区：[1]中国药科大学分析化学教研室,江苏南京210009 [2]江苏省环境科学研究院江苏省环境工程重点实验室,江苏南京210036

出　　处：《辽宁中医药大学学报》2010年第9期35-38,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家环保公益性行业科研专项项目(200809016-01);江苏省环境工程重点实验室开放基金项目(KF2009008)

摘　　要：目的:优化花生壳总黄酮的提取工艺,考察花生壳总黄酮抗氧化活性。方法:采用L9(34)正交实验,考察浸提剂浓度、料液比、浸提时间和温度等影响因素;研究D-101大孔吸附树脂分离富集花生壳提取液中总黄酮;参照Fenton反应原理研究花生壳总黄酮的清除.OH作用。结果:花生壳总黄酮的最佳提取工艺为:70%乙醇溶液为提取溶剂,采用料液比1:20(m:V),在80℃下浸提2h;D-101大孔吸附树脂能较好分离富集花生壳黄酮类物质;花生壳总黄酮对Fenton体系产生的.OH有较好的清除作用。结论:该研究为花生壳中有效成分的综合利用提供了实验基础。Objective：To study the extraction technology of total flavonoids from peanut hull and its antioxidizable activity.Methods：Concentration of extracting solution,solid-liquid ratio,temperature and time of extraction were investigated through orthogonal tests of L9（3^4）.In addition,D101 macroporous resin was used to enrich flavonoids and its scavenging of hydroxyl radicals by Fenton was also studied in the paper.Results：The optimum conditions of extracting flavonoids from peanut hull were：ethanol concentration 70%,solid-liquid ratio 1：2（0m：V）,extraction duration 2 h and refluxing temperature 80 ℃,respectively;D-101 macroporous adsorption resins can separate and purify flavonoids from peanut hull and flavanones from peanut hull had effects on scavenging of hydroxyl radicals.Conclusion：The study provides an experimental basis for the comprehensive utilization of active ingredient in peanut hull.

关 键 词：花生壳 总黄酮 提取 正交实验 清除自由基 

分 类 号：R282.6[医药卫生—中药学]