RNAi抑制胰腺癌PANC-1细胞MMP-2 mRNA的表达  

Inhibitory effect of RNA interference on expression of MMP-2 mRNA in pancreatic carcinoma cell line PANC-1

作　　者：朱学锋[1] 李德春[2] 陈益君[1] 许建伟[1] 顾继礼[1] 朱东明[2] 岑建农 

机构地区：[1]泰兴市人民医院肝胆外科,225400 [2]苏州大学附属第一医院普通外科 [3]江苏省血液研究所

出　　处：《江苏医药》2010年第16期1924-1927,共4页Jiangsu Medical Journal

基　　金：卫生部科学研究基金(WKJ2004-2-011)

摘　　要：目的观察RNAi体外沉默基质金属蛋白酶2(MMP-2)基因对胰腺癌PANC-1细胞MMP-2mRNA表达的抑制作用。方法设计靶向MMP-2的siRNA,并构建到pGCsi-U6质粒,重组质粒脂质体法转染PANC-1细胞;流式细胞术检测细胞GFP表达率,RQ-PCR检测细胞MMP-2的mRNA表达水平。结果 PCR鉴定证实重组质粒构建成功,质粒转染细胞GFP表达率最高达82.1%。转染干扰质粒的PANC-1细胞MMP-2基因mRNA表达抑制了71.74%(P<0.05)。结论 RNAi明显抑制胰腺癌PANC-1细胞MMP-2mRNA的表达,可望RNAi能为胰腺癌治疗开辟新的思路。Objective To investigate the inhibitory effects of RNA interference（RNAi） on the expression of matrix metalloproteinase-2 （MMP-2） mRNA of human pancreatic carcinoma cell line PANC-1 in vitro.Methods Small interference RNA（siRNA） targeting MMP-2 gene was designed and constructed to plasmid pGCsi-U6.Recombinant plasmids were transfected to pancreatic carcinoma cell line PANC-1 cells with lipofectamine.Green fluorescence protein（GFP） was evaluated by flow cytometry after transfection.RQ-PCR was used to detect the expression of MMP-2 mRNA.ResultsPCR confirmed that the MMP-2 siRNA plasmid was successfully constructed.The best efficiency of GFP after transfection was 82.1% in PANC-1 cells.After transfected by the MMP-2 siRNA plasmid,the MMP-2 mRNA expression of PANC-1 cells was suppressed by 71.74%（P0.05）.ConclusionRNAi targeting MMP-2 gene can obviously suppress the expression of MMP-2 mRNA of PANC-1 cells.RNAi is expected to open up a new approach to pancreatic carcinoma therapy.

关 键 词：胰腺癌 基质金属蛋白酶2 RNA干扰 

分 类 号：R735[医药卫生—肿瘤]