顺铂联合HMME介导的光动力疗法对人鼻咽癌CNE-2细胞杀伤作用的观察  

Cytotoxicity induced by cisplatin combined with HMME-based photodynamic therapy on human nasopharyngeal carcinoma cell line

作　　者：刘建中[1] 段艳芳[1] 李晓原[2] 刘佳[2] 李燕[3] 

机构地区：[1]中山大学中山医学院生物学教研室,广东广州510080 [2]中山大学中山医学院生物医学工程系激光医学研究室,广东广州510080 [3]中山大学中山医学院医学教学实验中心,广东广州510080

出　　处：《中华肿瘤防治杂志》2010年第14期1059-1062,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨顺铂(DDP)联合血卟淋单甲醚HMME介导的光动力学疗法(HMME-PDT)对人鼻咽癌细胞(CNE-2)的杀伤作用。方法:实验分对照组、单独DDP组、单独PDT组和联合组。所用的DDP浓度为0.8和1.6mg/L;光敏剂为HMME,剂量5mg/L,光能量密度为1.2、2.4和4.8J/cm2。联合组为不同剂量单独作用的组合。MTT法检测DDP和HMME-PDT单独或联合作用下24h的细胞抑制率,并用金氏公式分析联合效应。HE染色光镜下观察处理前后细胞的形态学变化,Hoechst染色荧光显微镜观察细胞核。结果:金氏公式分析显示,0.8和1.6mg/L的DDP与5mg/LHMME,1.2、2.4和4.8J/cm2能量密度的PDT,两者联合处理观察到协同或相加效应。HE和Hoechst染色均可见,联合组比单独处理组细胞形态学改变现象更明显。结论:DDP与HMME-PDT联合处理在一定剂量范围内可以协同杀伤CNE-2细胞。OBJECTIVE：To investigate the inhibitory effects of cisplatin combined with HMME-based photodynamic therapy （PDT） on human nasopharyngeal carcinoma cell lines （CNE-2） in vitro.METHODS：The experimental cells were divided into control group,PDT only group,DDP only group and combined treatment group.0.8 and 1.6 mg/L cisplatin were used to treat cells.The dose of HMME was 5 mg/L,the light doses were 1.2,2.4 and 4.8 J/cm^2.The inhibitory effects caused by the two treatments （cisplatin,HMME-PDT） administered individually and together were evaluated by MTT.Jin’s equation was used to evaluate the efficacy of drug combination.The morphological character 24 h post-treatment was examined by HE and Hoechst staining.RESULTS：Jin’s equation showed that 0.5 and 1.6 mg/L cisplatin combined with 5 mg/L HMME under 1.2,2.4 and 4.8 J/cm^2 energy dosage demonstrated additive or synergic effect.HE and Hoechst33342 stainning exhibited more obvious morphologic alterations in combined treatment group when compared to DDP and PDT group.CONCLUSION：Our study suggests that cisplatin combined with PDT has a significant synergistic inhibitory effect on CNE-2 cells in a certain range.

关 键 词：光动力学疗法 血卟啉单甲醚 顺铂 鼻咽肿瘤/药物疗法 联合作用 

分 类 号：R739.63[医药卫生—肿瘤]