外源性TGF-β1调控鼻咽癌细胞株凋亡的实验研究  

Regulation of exogenous TGF-β1 on apoptosis of nasopharyngeal carcinoma cell line

作　　者：孙蕊[1] 孙健[2] 陈明远[1] 华贻军[1] 曹卡加[1] 

机构地区：[1]华南肿瘤学国家重点实验室中山大学肿瘤防治中心鼻咽科,广东广州510060 [2]华南肿瘤学国家重点实验室中山大学肿瘤防治中心实验研究部,广东广州510060

出　　处：《中华肿瘤防治杂志》2010年第14期1063-1065,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：广东省医学科学技术研究基金(A2010188)

摘　　要：目的:应用体外实验研究探讨TGF-β1对鼻咽癌的作用机制。方法:应用免疫组化、MTT法和流式细胞仪技术,检测外源性TGF-β1对鼻咽癌细胞株生长、分化及凋亡等方面的影响。结果:TGF-β1受体TβRⅠ和TβRⅡ在鼻咽低分化CNE-2癌细胞株中均有表达,进一步给予梯度浓度的外源性TGF-β1作用于鼻咽癌CNE-2细胞株,应用MTT法发现外源性TGF-β1与对照组相比,能显著抑制CNE-2癌细胞株的生长,且最小显效浓度为0.1ng/mL,再用流式细胞仪进行检测,发现外源性TGF-β1作用后出现G1期阻滞、S期和G2/M期比例下降,凋亡比例则随着时间的延长逐渐增高且与对照组比较差异均有统计学意义。结论:TGF-β1/Smad信号传导通路可以通过调控鼻咽癌细胞的生长、细胞周期和凋亡等活动,从而参与鼻咽癌的发生和发展。OBJECTIVE：To detect the mechanism of TGF-β1 signaling pathway on the CNE-2 cell line.METHODS：By using immunohistochemical staining,MTT assay and Flow cytometry,the proliferation,differentiation and apoptosis of exogenous TGF-β1 on Nasopharyngeal carcinoma cell line were detected.RESULTS：The expression of TβRⅠ and TβRⅡ were all expressed in low differentiated Nasopharyngeal carcinoma cell line CNE-2.The exogenous TβRⅠ could inhibited the growth of CNE-2 cell line.The minimum valid concentration of exogenous TGF-β1 is 0.1 ng/mL.This study also showed G1,S and G2/M ratios were blocked,but apoptosis ratio increased and depended on time,and there was no significant difference with the controls.CONCLUSION：TGF-β1/Smad signaling pathway could play a great role in proliferation,cell cycle and apoptosis during oncogenesis and progression of NPC.

关 键 词：鼻咽肿瘤 转化生长因子 分化 凋亡 

分 类 号：R739.63[医药卫生—肿瘤]