非小细胞肺癌组织MGMT基因启动子过甲基化与临床病理特征相关性的研究  

Promoter hypermethylation of MGMT gene and clinicopathologic in human non-small cell lung cancer tissues

作　　者：刘莹[1] 王静[1] 吴逸明[2] 吴拥军[2] 

机构地区：[1]郑州大学第一附属医院呼吸科,河南郑州450052 [2]郑州大学公共卫生学院,河南郑州450002

出　　处：《中华肿瘤防治杂志》2010年第14期1077-1079,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30571552);河南省科技攻关项目(82300450240)

摘　　要：目的:探讨非小细胞肺癌(NSCLC)组织中DNA修复酶O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)过甲基化状况与临床病理特征的关系。方法:甲基化特异性PCR(MSP)检测120例病理确诊的非小细胞肺癌组织及癌旁正常肺组织和30例病理确诊为肺炎症组织中MGMT的甲基化状况。结果:120例NSCLC组织中MGMT基因启动子甲基化率(35%)显著高于癌旁正常组织甲基化率(5%,P<0.05),亦显著高于30例正常肺组织中甲基化率(0,P<0.05)。NSCLC中MGMT基因启动子过甲基化状态与淋巴结转移、TNM分期、吸烟史、肿瘤分化程度有关(P值均<0.05),与患者年龄、性别、病理组织类型无关(P值均>0.05)。结论:在NSCLC中,MGMT基因启动子甲基化可能与肺癌的发生、发展相关。OBJECTIVE：To investigate the relationgship between the promoter hypermethylation of MGMT gene and clinicopathologic feature in non-small cell lung cancer（NSCLC） tissues.METHODS：Methylation specific of 120 NSCLC and 30 pneunomia tissues was detected by methylation specific PCR（MSP）.RESULTS：The rate of the promoter hypermethylation of MGMT gene in lung cancers was 35%,significant higher than that in adiacent normal lung tissues（5%,P〈0.05）,and 30 infection lung tissues（0,P〈0.05）.There was significant correlation was found between the promoter hypermethylation of MGMT and TNM stage,lymph node metastasis,degree of tumor differation,smoking（P〈0.05）.And no significant correlation was found between the promoter hypermethylation of MGMT and age,gender,histology（P〈0.05）.CONCLUSION：The promoter hypermethylation of MGMT gene may take part in lung carcinoma evolvement.

关 键 词：癌 非小细胞肺 O6-甲基鸟嘌呤-DNA甲基转移酶 甲基化 

分 类 号：R734.2[医药卫生—肿瘤]