壮骨镇痛胶囊对MDA-MB-231细胞骨架蛋白表达的影响  

Effects of Zhuanggu Zhentong capsules on expression of cytoskeletal proteins of breast cancer cell lines MDA-MB-231 in vitro

作　　者：柳景红[1] 何迎春[2] 曹建雄[1] 田道法[2] 卓耀[1] 何欣[1] 

机构地区：[1]湖南中医药大学附属第一医院肿瘤科,湖南长沙410007 [2]湖南中医药大学中西医结合学院循证医学教研室中医五官科学省级重点学科,湖南长沙410007

出　　处：《中华肿瘤防治杂志》2010年第15期1164-1167,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：湖南省科技厅资助项目(2007TP4021和湘财教指2009-42);湖南省教育厅资助项目(08C637和09C724)

摘　　要：目的:研究壮骨镇痛胶囊对高转移人乳腺癌MDA-MB-231细胞骨架蛋白p-Ezrin、F-actin和β-tubulin表达水平的影响。方法:RT-PCR法检测不同浓度壮骨镇痛胶囊含药血浆对MDA-MB-231细胞p-Ezrin基因转录水平的影响,免疫细胞化学法检测其对MDA-MB-231细胞p-Ezrin、F-actin和β-tubulin翻译水平的影响。结果:5.0%、1.25%壮骨镇痛胶囊含药血浆显著抑制了MDA-MB-231细胞p-Ezrin基因的转录(P值分别为0.000和0.042)和翻译(P值分别为0.000和0.001),抑制了F-actin(5.0%和1.25%的P值分别为0.003和0.044)和β-tubulin(5.0%和1.25%的P值均为0.000)蛋白表达;0.63%壮骨镇痛胶囊含药血浆反而促进了p-Ezrin基因的转录(P=0.000),但对p-Ezrin、F-actin和β-tubulin蛋白表达无显著影响。结论:壮骨镇痛胶囊可通过抑制乳腺癌MDA-MB-231细胞p-Ezrin、F-actin和β-tubulin蛋白表达来降低细胞的迁移能力,并且该作用与浓度密切相关。OBJECTIVE：To study the effects of Zhuanggu Zhentong capsules （ZZC） on the expression of cytoskeletal proteins such as phos-Ezrin,F-actin and β-tubulin of breast cancer cell lines MDA-MB-231 in vitro.METHODS：The transcriptions of phos-Ezrin in MDA-MB-231 cells treated with different concentrations of ZZC contained plasma were examined by reverse transcriptase PCR protocol （RT-PCR）.These cytoskeletal proteins were assayed by immunocytochemistry protocol.RESULTS：The expressions of mRNA （P were 0.000 and 0.042） and proteins of phos-Ezrin （P were 0.000 and 0.001） in the MDA-MB-231 cells treated with 5.0% ZZC contained plasma and 1.25% ZZC contained plasma were inhibited significantly.F-actin and β-tubulin proteins were also inhibited significantly in the cells treated with 5.0% ZZC and 1.25% ZZC contained plasma （F-actin：P were 0.003 and 0.044;β-tubulin：both P were 0.000）.The expressions of phos-Ezrin mRNA significantly improved （P=0.000） in MDA-MB-231 cells troated with 0.63% ZZC contrained plasma.But there were no obvious effects on phos-Ezrin,F-actin and β-tubulin proteins in the MDA-MB-231 cells treated with 0.63% ZZC contained plasma.CONCLUSION：ZZC contained plasma can inhibit the migrations of MDA-MB-231 cells in a dose-dependent manner by inhibiting the expressions of cytoskeletal proteins such as phos-Ezrin,F-actin and β-tubulin.

关 键 词：乳腺肿瘤 中草药 微管蛋白质类 肌动蛋白类 

分 类 号：R737.9[医药卫生—肿瘤]