骨髓CD34+细胞在肝硬化患者中的表达及意义  

Signif icance of bone marrow CD34+ cell levels in cirrhotic patients

作　　者：刘拉羊[1] 王顺达[1] 党双锁[1] 程延安[1] 周芙玲[2] 高宁[1] 贾晓黎[1] 张欣[1] 

机构地区：[1]西安交通大学医学院第二附属医院感染科,陕西省西安市710004 [2]西安交通大学医学院第二附属医院血液科,陕西省西安市710004

出　　处：《世界华人消化杂志》2010年第20期2155-2158,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30670961~~

摘　　要：目的:探讨肝硬化患者骨髓CD34+造血干细胞的表达及意义.方法:行骨髓穿刺术,收集31例肝硬化患者和15例健康对照的骨髓液,流式细胞仪检测骨髓液CD34+细胞占单个核细胞比例,并比较随肝脏储备功能不同的ChildA、ChildB、ChildC级患者,其骨髓CD34+细胞表达水平是否存在差异.结果:肝硬化患者和健康对照组骨髓CD34+细胞占单个核细胞的比例分别为6.30%和1.87%,差异有统计学意义(t=3.906,P<0.05).随肝脏储备功能不同的ChildA、ChildB、ChildC级患者,其骨髓CD34+细胞表达率分别为7.01%±2.1%、4.58%±2.56%、7.72%±1.49%,三者之间差异无统计学意义(F=3.586,P>0.05).结论:肝硬化患者骨髓CD34+干细胞细胞增加,随肝功能Child-Pugh分级不同CD34+干细胞表达无差异,CD34+增加可能对肝硬化的病情有积极的作用.AIM：To analyze the signif icance of bone marrow CD34＋ levels in cirrhotic patients. METHODS：Bone marrow fluid was obtained from 31 cirrhotic patients and 15 healthy controls.The percentage of bone marrow CD34＋ cells among mononuclear cells was detected by flow cytometry. RESULTS：The percentage of bone marrow CD34＋ cells among mononuclear cells was significantly higher in cirrhotic patients than in normal controls（6.30% vs 1.87%,t = 3.906,P〈 0.05）.The percentages of bone marrow CD34＋ cells were not signif icantly different among patients with Child-Pugh A,B,and C cirrhosis（7.01 ± 2.1%,4.58 ± 2.56% and 7.72 ± 1.49%,F = 3.586,P〉 0.05）. CONCLUSION：The levels of bone marrow CD34＋ cells increase in cirrhotic patients.Liver function grade is not correlated with bone marrow CD34＋ cell levels in cirrhotic patients.Increased bone marrow CD34＋ cells may contribute to the pathogenesis of cirrhosis.

关 键 词：肝硬化 CD34+造血干细胞 CHILD-PUGH分级 

分 类 号：R575.2[医药卫生—消化系统]