脂蛋白在脂多糖诱导THP-1细胞活化中的调节机制  

Regulatory mechanism of lipoprotein on LPS-induced monocyte activation

作　　者：舒旷怡[1] 李方去[1] 杨锦红[1] 杨爱平[1] 陶洪群[1] 陈慧[1] 李向阳[1] 

机构地区：[1]温州医学院附属第二医院检验科,325027

出　　处：《国际流行病学传染病学杂志》2010年第4期244-247,共4页International Journal of Epidemiology and Infectious Disease

基　　金：浙江省自然科学基金（Y2080596）

摘　　要：目的 研究血清脂蛋白对脂多糖的影响机制,并对不同种类脂蛋白：高密度脂蛋白（HDL）、低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）,抑制脂多糖的能力进行比较,为研究脂蛋白在治疗败血症中的应用提供理论基础.方法 通过检测经佛波醇酯刺激分化的THP-1细胞分泌的TNF-α间接反映脂蛋白对脂多糖的抑制作用.将不同浓度脂多糖与THP-1细胞在血清基质中混合孵育4 h,并与3种脂蛋白-脂多糖混合液与THP-1细胞混合孵育后TNF-α的分泌量进行对比,计算脂蛋白对脂多糖的抑制情况.结果 HDL、LDL和VLDL单独不能诱导分化的THP-1细胞分泌TNF-α（P＞0.05）.当10 ng/mL脂多糖与0.05 mg/m以上浓度的脂蛋白（HDL、LDL和VLDL）混合时,TNF-α分泌量明显降低.当脂多糖浓度为100 ng/mL时,0.1 mg/mL的脂蛋白（HDL、LDL和VLDL）对其抑制效果较低,分别为（20.7±11.1）%、（8.9±2.8）%、（4.3±1.6）%;在脂多糖浓度为10 ng/mL抑制效果明显增强,抑制率分别为（69.3±3.7）%、（42.9±5.7）%、（42.7±4.5）%.HDL对各浓度脂多糖的抑制效果均强于LDL和VLDL（P＜0.05）.结论 脂蛋白在血清中能抑制脂多糖活化巨噬细胞分泌细胞因子,但抑制效果在脂多糖浓度高时相比浓度低时差.HDL对脂多糖活化巨噬细胞的抑制作用比LDL和VLDL强.Objective To investigate the affect mechanism of serum lipoproteins（HDL, LDL, VLDL） on LPS,and compare the regulation ability in LPS-activated of them, to provide theoretical basis for the application of lipoprotein in treatment of sepsis. Methods TNF-α secreted from PMA-differentiated THP-1 cell were detected to reflect the inhibitory action of lipoprotein on LPS indirectly. THP-1 cell were incubated with LPS for 4 houris, TNF-α levels were detected and then compared with the levels in which THP-1 cell incubated with lipoprotein-LPS mixture. Futhermore, the inhibitory action from lipoprotein to LPS was estimated. Results HDL, LDL and VLDL did not induce differentiated THP-1 cells to secrete TNF-α（ P 〉 0.05）. When lipoprotein was above 0.05 mg/mL and mixed with 10 ng/mL LPS,TNF-α decreased obviously. The inhibition action of 0. 1 mg/mL lipoprotein （HDL, LDL, VLDL） on LPS was low when LPS was 100 ng/mL, and the rates were （20.7± 11.1）%, （8.85＋2.75）%, （4.3± 1.6）% respectively; the inhibitory effect was significantly increased when LPS was 10 ng/mL, and the rates were （69.3±3.7）%, （42.9±5.7）%,（42.7±4.5）% respectively. HDL had stronger inhibitory effect than LDL and VLDL（P 〈 0.05）. ConclusionsLipoprotein in serum can inhibit LPS-activated macrophage cells to secrete cytolines, but the inhibition of lipoprotein is more obvious in high concentration of LPS than low concentration. HDL has a stronger inhibitory effect on LPS activation than LDL and VLDL.

关 键 词：脂多糖类 脂蛋白 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]