抗阻训练对小鼠骨骼肌DNA损伤、脂质过氧化及抗氧化能力的影响  

Effects of anti-resistance training on the DNA damage, lipid peroxidation and anti-oxidation capacity of skeletal muscles of mice

作　　者：王立丰[1] 李海鹏[2] 王静[1] 马景亮[1] 陈彩珍[1] 卢健[1] 

机构地区：[1]华东师范大学体育与健康学院,上海200241 [2]中国矿业大学体育学院,江苏徐州221116

出　　处：《体育学刊》2010年第8期107-111,共5页Journal of Physical Education

基　　金：教育部博士点基金项目(200802690017)

摘　　要：采用负重爬梯模型进行抗阻训练,观察年龄和抗组训练对SAMP-8小鼠体重、SI及骨骼肌氧化应激的影响。40只雄性SAMP-8小鼠随机分成青年安静组(YC)、青年抗阻组(YR),老年安静组(OC)和老年抗阻组(OR)。抗阻训练组进行8周爬梯运动,每周3次。通过比色法检测T-AOC、SOD活性和MDA含量,ELISA试剂盒测定8-OH-dG的含量。结果:各组的体重均没有明显的差异,而YC组的SI明显高于OC组(P<0.05),其它各组之间没有明显的差异;YC组总SOD活性和T-AOC高于OC组(P<0.05),YR组的总SOD活性和T-AOC明显低于YC组(P<0.05),而OR组的总SOD活性高于OC组(P<0.05),T-AOC却与之相反(P<0.05);OC组的8-OH-dG的质量浓度明显高于YC组(P<0.05),YC组与YR组的8-OH-dG的浓度无显著差异,而OR组的8-OH-dG的质量浓度明显低于OC组(P<0.05),YC组MDA的质量摩尔浓度低于OC组(P<0.05),YR组和OR组MDA的质量摩尔浓度低于YC组和OC组(P<0.05)。结果说明:1)在衰老过程中,SAMP-8小鼠的体重未发生明显的变化,SI却有明显的变化,表现出了骨骼肌衰减的特征,而抗阻运动对于体重、骨骼肌质量的改变均没有影响。2)衰老时,骨骼肌的抗氧化水平明显地降低,而氧化损伤却显著增高,通过抗阻训练可以明显逆转这一状况。By using a load bearing ladder climbing model to conduct anti-resistance training,the authors observed the effects of age and anti-resistance training on the weight,SI and skeletal muscle oxidation stress of SAMP-8 mice.40 male SAMP-8 mice were randomly divided into a youth calm（YC） group,a youth anti-resistance（YR） group,an old calm（OC） group,and an old anti-resistance（OR） group.The anti-resistance groups performed ladder climbing for 8 weeks,3 times a week.The authors measured the T-AOC,SOD activity and MDA content by means of colorimetry,and measured the content of 8-OH-dG by using an ELISA kit.Results： there was no significant difference in weight between mice in various groups,while the SI of mice in the YC group was significantly higher than that of mice in the OC group（P0.05）,and there was no significant difference in SI between mice in other groups;the total SOD activity and T-AOC of mice in the YC group were higher than those of mice in the OC group（P0.05）,the total SOD activity and T-AOC of mice in the YR group were significantly lower than those of mice in the YC group（P0.05）,while the total SOD activity of mice in the OR group was higher than that of mice in the OC group（P0.05）,yet the T-AOC of mice in the OR group was lower than that of mice in the OC group（P0.05）;the concentration of 8-OH-dG of mice in the OC group was significantly higher than that of mice in the YC group （P0.05）,and there was no significant difference in the concentration of 8-OH-dG of mice in the YC group and YR group,while the concentration of 8-OH-dG of mice in the OR group was significantly lower than that of mice in the OC group（P0.05）,the MDA concentration of mice in the YC group was lower than that of mice in the OC group （P0.05）,and the MDA activity of mice in the YR group and OR group was lower than that of mice in the YC group and OC group（P0.05）.The results indicated the followings： 1） in the process of aging,there was no significant change to the weight of SAMP-

关 键 词：运动生物化学 骨骼肌衰减征 抗阻训练 DAN损伤 8羟基脱氧鸟苷 

分 类 号：G804.4[文化科学—运动人体科学]