荷瘤大鼠射频消融治疗前后肝脏巨噬细胞炎性蛋白-1α的变化及其意义  

Changes of macrophage inflammatory protein-1α in the livers of rats with Walker-256 tumors treated with radiofrequency ablation

作　　者：戴维德[1] 范智慧[2] 陈敏华[2] 河福金[3] 李洪民[3] 

机构地区：[1]卫生部北京医院超声科,北京100730 [2]北京大学临床肿瘤学院超声科 [3]北京大学临床肿瘤学院病理科

出　　处：《中华物理医学与康复杂志》2010年第8期574-577,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：北京市科委重大项目培育专项（Z0005190040431）

摘　　要：目的 研究射频消融（RFA）治疗大鼠肝肿瘤前、后肝脏局部组织内单个核细胞中OX-62、OX-6及CD86阳性表达率及其相关趋化因子巨噬细胞炎性蛋白1α（MIP-1α）的变化,探讨RFA对肝脏局部组织内树突状细胞分化迁移的影响.方法 取60只正常Sprague Dawley（SD）大鼠制作Walker-256肝肿瘤模型后,分为RFA后7 d组、RFA后14 d组和荷瘤对照组,每组20只.RFA后7 d组、RFA后14 d组分别于RFA处理后第7天及第14天处死,对照组不做RFA处理即处死.取消融灶周边（对照组取肿瘤周边）0.5 cm范围内肝组织,采用Ficoll密度梯度离心法分离出单个核细胞,应用流式细胞仪检测单个核细胞OX-62、OX-6及CD86表达水平.采用酶联免疫吸附测定（ELISA）法检测肿瘤周边或消融灶周边0.5 cm范围内肝组织内MIP-1α浓度.结果 荷瘤对照组肿瘤周边肝组织内单个核细胞中有（15.29±4.59）%表达OX-6,RFA后7 d组和RFA后14 d组OX-6阳性表达率分别为（34.20±11.62）%、（39.18±9.14）%,与荷瘤对照组相比差异均有统计学意义（P＜0.05）.荷瘤对照组肿瘤周边肝组织内单个核细胞中有（18.91±4.58）%表达OX-62,RFA后7 d组和RFA后14 d组OX-62阳性表达率分别为（24.49±4.45）%、（22.77±3.50）%,RFA后7 d组与荷瘤对照组相比差异有统计学意义（P＜0.05）.荷瘤对照组肿瘤周边肝组织内单个核细胞中有（66.29±17.69）%表达CD86,RFA后7 d组CD86阳性表达率为（55.29±10.69）%,RFA后14 d组为（55.93±12.64）%,2组与荷瘤对照组比较差异均无统计学意义（P＞0.05）.荷瘤对照组肿瘤周边肝组织内MIP-1α浓度为（232.92±54.58）pg/ml,RFA后7 d组和RFA后14 d组分别为（499.38±15.14）pg/ml、（495.90±9.94）pg/ml,与荷瘤对照组相比差异均有统计学意义（P＜0.05）.结论 RFA治疗后荷瘤大鼠肝脏MIP-1α表达水平增高,促进树突状细胞的迁移,而肝脏单个核细胞OX-62、OX-6及CD86阳性表达率的变化也反映出这类Objective To study changes in the expression levels of OX-62, OX-6 and CD86 of mononuclear cells and the related chemotatic factor macrophage inflammatory protein-1α （MIP-1α） in the livers of rats with Walker-256 tumors treated with radiofrequency ablation （RFA） and to elucidate the influence of RFA on differentiation and migration of liver dendritic cells（DCs）.Methods Walker-256 liver tumors were induced in 60 SpragueDawley rats by implanting tumor particles. These rats were randomly divided equally into three groups from which liver tissue around the local area of the tumor was sampled at 7 d and 14 d after RFA. The mononuclear liver cells were separated with Ficoll density gradient centrifugation. The expression levels of OX-62, OX-6 and CD86 in the mononuclear cells were analyzed with flow cytometry. The expression level of MIP-1α in the liver tissue was detected by enzyme-linked immunosorbent assay （ELISA）. Results The average expression of OX-6 in the control rats was 15.29 ±4.59% and those 7 d and 14 d after RFA were 34.20±11.62% and 39.18±9.14% respectively. The difference between the two RFA groups and the control group was statistically significant. The average expression rate of OX-62 in the control rats was 18.91±4.58% and those 7 d and 14 d after RFA were 24.49±4.45% and 22.77 ± 3.50% respectively. The difference between the 7 d group and the control group was significant. The average expression rate of CD86 in the control rats was 66.29±17.69% and those 7 d and 14 d after RFA were 55.29±10.69% and 55.93±12.64% respectively. These differences between both RFA groups and the controls group were not significant. The average expression level of MIP-1 α around the tumors was 232.92±54.58 pg/ml in the controls and 499.38±15.14 pg/ml and 495.90±9.94 pg/ml 7d and 14 d after RFA respectively. These differences from the controls were both statistically significant. Conclusion The expression of MIP-1α around the tumors was elevated after RFA, which could promote the migration o

关 键 词：射频消融 大鼠 肝肿瘤 树突状细胞 巨噬细胞炎性蛋白-1Α 迁移 

分 类 号：R735.7[医药卫生—肿瘤]