谷氨酸对神经细胞凋亡的影响  

Glumatic acid and neurocyte apoptosis

作　　者：陈世伟[1] 张峰[1] 张黎明[1] 

机构地区：[1]中国医科大学附属第四医院,110032

出　　处：《山西医药杂志（上半月）》2010年第9期801-803,共3页Shanxi Medical Journal

基　　金：辽宁省科技厅攻关项目(2007225016)

摘　　要：目的研究谷氨酸对神经细胞的毒性作用及其影响程度。方法随机取雄性兔抗大鼠14只,分别制备生理盐水对照组(A6-A1),及谷氨酸(C6-C1)6 h,24 h,3 d,5 d,7 d动物模型。分别行原位交叉免疫组织化学染色和TUNEL染色,观察谷氨酸在不同时间点对神经细胞凋亡的影响。结果谷氨酸组引起神经细胞凋亡数与生理盐水对照组各时间点比较差异均有统计学意义(P均<0.05)。结论一定剂量的谷氨酸可引起神经细胞凋亡。Objective To study the toxic effect of glumatic acid on nerve cell of Sprague-Dawley rat by injecting different concentration glumatic acid and to determine the degree of nerve cell apoptosis in different time by using immunohistochemistry technique.Methods Seting SD rat model and observing the toxic effect of glumatic acid on nerve cell at different time by using cross immunity immunohistochemistry technique in situ and TUNEL staining method.Results There was remarkably difference between glumatic acid group and the normal sodium group by counting the number of nerve cell apoptosis（P0.05）.Conclusion Specified dosage of glumatic acid can cause nerve cell apoptosis.

关 键 词：谷氨酸 细胞凋亡 神经细胞 

分 类 号：R743.34[医药卫生—神经病学与精神病学]