亚砷酸对人胰腺癌PANC-1细胞增殖及RASSF1A基因甲基化的影响  

Effect of arsenic trioxide on proliferation and RASSF1A gene methylation of human pancreatic cancer PANC-1 cells

作　　者：朱应昌[1,2] 包仕廷[1] 邱志东[1] 郭日昌[1] 陈晖宽[1] 

机构地区：[1]广东医学院附属医院肝胆外科,广东湛江524001 [2]广东省佛山市南海区人民医院普外一科,广东佛山528200

出　　处：《广东医学院学报》2010年第4期359-361,共3页Journal of Guangdong Medical College

摘　　要：目的探讨亚砷酸对人胰腺癌PANC-1细胞增殖及RAS相关结构域家族基因1A(RASSF1A)甲基化的影响。方法 PANC-1细胞用不同浓度亚砷酸处理,采用MTT法检测细胞增殖,甲基化特异性PCR(MSP)法检测RASSF1A基因甲基化情况。结果随着亚砷酸浓度增加(1.2～4.8μmol/L)和作用时间延长,PANC-1细胞抑制率逐渐增加(P<0.05)。PANC-1细胞中RASSF1A基因表现为高甲基化状态,但其甲基化随亚砷酸浓度增加而逐渐减弱。结论亚砷酸对PANC-1细胞增殖的抑制作用具有时间和剂量依赖性,并通过RASSF1A基因的去甲基化起作用。Objective To investigate the effect of arsenic trioxide on the proliferation and RAS association domain family gene 1A （RASSF1A） methylation of human pancreatic cancer PANC-1 cells. Methods PANC-1 cells were treated with different concentra-tions of arsenic trioxide, and their proliferation and RASSF1A gene methylation were then detected by MTT and methylation specific PCR （MSP）, respectively. Results The inhibition rate of PANC-1 cells augmented gradually with the increase of arsenic trioxide concentration （1.2- 4.8 μmol/L） and duration （P〈0.05）. There was an upregulation of RASSF1A gene methylation in PANC-1 cells. However, RASSF1A gene methylation decreased gradually with the increase of arsenic trioxide concentration. Conclusion Arsenic trioxide can inhibit the proliferation of PANC-1 cells in a time and dose dependent manner by demethylation ofRASSF1A gene.

关 键 词：胰腺癌 亚砷酸 RASSF1A 甲基化 

分 类 号：R735.9[医药卫生—肿瘤]