负载辅酶Q10的纳米结构脂质载体的制备及理化性质研究  

Research on Preparation and Physicochemical Characteristics of CoQ10-loaded Nanostructured Lipid Carriers

作　　者：王虹霞[1,2,3] 夏强[1,2,3] 徐弟[1,2,3] 

机构地区：[1]东南大学生物电子国家重点实验室,江苏南京210096 [2]东南大学苏州市生物医用材料与技术重点实验室,江苏苏州215123 [3]苏州纳康生物科技有限公司,江苏苏州215123

出　　处：《纳米科技》2010年第4期24-27,共4页

基　　金：科技部国际科技合作项目（2008DFB50060）; 苏州市科技型企业技术创新资金专项（SG0958）

摘　　要：运用高压均质法制备出负载辅酶Q1（0CoQ10）的纳米结构脂质载体（NLC）,利用光子相关光谱（PCS）测得在常温下的平均粒径为108±10nm,多分散性指数（PI）在0.2-0.6之间;Zeta电位是-28±5mV;随着负载量的增加,NLC的粒径明显增大,高压均质的次数增多,NLC的粒径减小。利用pH计测得样品的pH接近于7.0。从温度和时间稳定性、离心稳定性两个方面对CoQ10-NLC的物理稳定性进行研究,测得在常温下避光保存的CoQ10-NLC的粒径变化较小,低温下易凝聚沉淀;在10000r/min的转速下离心制备得到的NLC分散液25min不分层。最后运用反相高效液相色谱法测得CoQ10-NLC的含量4.709g·mL^-1。CoQ10-loaded Nanostructured Lipid Carriers were prepared by high Press Homogenization （HPH）.The average particle sizes measured by Photon Correlation spectroscopy（PCS）were in the range of 108 nm±10 nm, and the poly-dispersity index （PI）were from 0.2 to 0.6.The zeta potential values were-28 ±5 mV.The particle size becomes bigger with increasing the content of CoQ10 loaded in NLC, however, the size of CoQ10-NLC became smaller because of the in-crease of the number of cycle of High Pressure Homognization.The pH of CoQ10-NLC near to 7.0 was close to the pH of skin of man.The CoQ10-loaded NLC delaminated difficultly after high-speed centrifugal effect in different temperatures and different time by High-Speed Centrifuge.And, the particle size had only a little change at room temperature.The content of CoQ10-NLC is 4.709g·mL^-1 by RP-HPLC.

关 键 词：辅酶Q10 纳米结构脂质载体 稳定性 含量 

分 类 号：TB34[一般工业技术—材料科学与工程]