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作 者:侯雁[1] 冼小敏[1] 林洁茹[1] 赖小平[1] 陈建南[1]
机构地区:[1]广州中医药大学中药学院,广东广州510405
出 处:《中国当代医药》2010年第26期9-11,共3页China Modern Medicine
摘 要:目的:研究燕窝对ConA刺激下大鼠淋巴细胞转化的促进作用。方法:为了建立大鼠淋巴细胞转化检测的MTT法,设计了细胞浓度、ConA浓度和培养时间3个参数;考察不同品种燕窝以及伪品对淋巴细胞转化的作用。结果:最佳条件为,培养时间48h,ConA最佳浓度2μg/ml,细胞浓度2×106个/ml,燕窝浓度40μg/ml。结论:燕窝对大鼠淋巴细胞无直接转化作用,但在低浓度ConA刺激下对大鼠淋巴细胞的转化有辅促作用。Objective:To research on the ConA induced lymphocytes transformation of rats and its promoting effect stimulated by Edible birds' nest (short for EBN). Methods:Chose three parameters:cell concentration,ConA concentration and culture period to establish MTT assay,then detected lymphocytes transformation of rats by this method; Studied on the ef -fect of lymphocytes transformation stimulated by Edible Birds' Nest and its counterfeits. Results:The optimum conditions were certified,cell conditon 2×10^6/ml,ConA concentration 2 μg/ml,culture period 48 h and Edible birds' nest concentra-tion 40 μg/ml. Conclusion:This study show that Edible birds' nest had no effect to stimulate lymphocytes transformation of rats directly,but with induction of low concentration of ConA,Edible birds' nest showed transformation effect to rats' lymphocytes.
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