鸡蛋清中溶菌酶提取技术的研究  

Study on the Extraction of Lysozyme in Egg-white

作　　者：迟晓艳[1] 葛宜和[1] 韩立亚[1] 侯月利[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《湖北农业科学》2010年第9期2212-2214,共3页Hubei Agricultural Sciences

基　　金：鲁东大学校基金项目(L20073302)

摘　　要：对鸡蛋清中溶菌酶的提取及纯化技术进行探究,从而获得纯度、活性、浓度均较高的溶菌酶。采用弱酸性阳离子交换树脂法提取,离心透析纯化,SDS-聚丙烯酰胺凝胶电泳检测。结果表明,用此方法提取的溶菌酶纯度较高,酶活力为7793U/mg。To study the extraction and purification technology of egg-lysozyme,then to obtain higher purity,activity and concentration of lysozyme.Weak acidic cation exchange resin was used to extract,centrifugal dialysis and SDS-polyacrylamide gel electrophoresis was used to purify and detect.The purity of lysozyme was high with this method,and the activity of lysozyme was 7 793U/mg.

关 键 词：鸡蛋清 溶菌酶 724树脂再生 阳离子交换树脂 

分 类 号：Q556.2[生物学—生物化学]