吴茱萸碱对人胃癌SGC7901细胞增殖、侵袭的影响及机制探讨  

Effects of evodiamine on invasion and proliferation in human gastric cancer cell

作　　者：祁卫东[1] 周永静[1] 范钰[1] 

机构地区：[1]江苏大学附属人民医院,江苏镇江212002

出　　处：《山东医药》2010年第34期25-26,共2页Shandong Medical Journal

基　　金：江苏省社会发展基金资助项目(BS2006041)

摘　　要：目的观察吴茱萸碱对胃癌细胞增殖、侵袭的影响,并探讨其机制。方法采用不同浓度的吴茱萸碱处理人胃癌细胞SGC7901,用软琼脂集落培养试验检测癌细胞增殖情况,Boyden小室检测癌细胞侵袭能力;分别采用荧光实时定量RT-PCR法和Western blot法检测癌细胞中的中期因子(MK)mRNA及其蛋白。结果 SGC7901经吴茱萸碱处理后,恶性增殖和穿膜细胞数均明显下降,且呈剂量依赖性(P均<0.05);吴茱萸碱处理后,SGC7901的MK mRNA及蛋白表达均明显下调,且呈时间、浓度依赖性(P均<0.01)。结论吴茱萸碱可明显抑制胃癌细胞的增殖及侵袭能力,其机制可能与下调MK基因表达有关。objective To study the effect and mechanism of evodiamine on invasion and proliferation in human gastric cancer cell.Methods Human gastric cancer SGC7901 cell were treated with different doses of evodiamine,and the growth of anchorage independence of cancer cell was studied by colony formation in soft agar,and invasion ability were determined by Boyden chamber,and the level of midkine gene mRNA and protein were detected by real time RT-PCR and Western blot assay,respectively.Results Evodiamine could significantly inhibit both invasion ability and anchorage independence growth in dose-dependent manners.The level of mRNA and protein of midkine of cancer cells treated with evodiamine reduced in time-and dose-dependent manners.Conclusion Evodiamine can inhibit invasion of gastric carcinoma cell through down-regulating of midkine expression.

关 键 词：胃肿瘤 吴茱萸碱 侵袭 中期因子 

分 类 号：R735.2[医药卫生—肿瘤]