VEGF在腹膜透析模型大鼠中的表达及意义  

Significance on the Expressions of VEGF in Peritoneal Dialysis Rat Model

作　　者：郭小云[1] 夏天[1] 

机构地区：[1]天津医科大学第二医院肾内科,天津300211

出　　处：《河北北方学院学报（医学版）》2010年第4期8-11,共4页Journal of Hebei North University:Medical Edition

摘　　要：目的:观察在非感染状态下腹膜透析大鼠模型腹膜组织的病理变化,测定腹膜间皮细胞血管内皮生长因子(VEGF)表达水平,探讨VEGF在腹膜透析大鼠腹膜组织中的表达。方法:应用ELISA法测定CAPD大鼠血清及腹透放出液中VEGF的含量,免疫组织化学法检测VEGF蛋白在腹膜透析大鼠腹膜组织中的表达。结果:随着腹透液浓度增高,腹膜间皮细胞VEGF的表达增强、阳性面积增大;正常对照组未见VEGF的表达。4.25%腹透液组的腹腔透出液VEGF蛋白质水平明显高于1.5%和2.5%透析液组,差异均有显著性(P<0.05)。四组中血清VEGF蛋白质水平相近,组间比较差异均无显著性(P>0.05)。结论:腹膜透析液葡萄糖浓度的升高可以导致大鼠腹膜表达VEGF的升高,高糖腹膜透析液能通过增加大鼠腹膜中VEGF的合成促进腹膜的纤维化。提示高浓度腹透液可能通过腹膜间皮细胞对VEGF表达和分泌产生影响,促使腹膜滤过功能的减退。Objective：To investigate the pathological change of peritonium in non--infection situations, observe VEGF expression changes PMC in short-term period by rat CAPD models,and investigate the effects of VEGF producted by PMC as well as the associations with CAPD related peritoneal sclerosis. Methods：Dialysate samples and serum of CAPD rat models were collected to measure VEGF protein levels by ELISA kit and parietal peritoneum tissue was taken to determine VEGF expression by IHC. Results：VEGF protein expression in parietal peritoneum tissue was significantly increased along with the increased PD density, there was no VEGF protein expression in control group. The VEGF protein expression was significantly increased in 4.25% PD group compared with that of 1.5%PD group and 2.5%PD group（P〈0.05）. VEGF protein levels of PDF were increased in 4.25 %group compared with that of control group 1.5% and 2.5% group,and the difference had statistic significance（P〈0.05）. VEGF protein levels of serum in whole 4 groups showed no significant differenee（P〉0.05）. Conclusion： PDF could induced increase of VEGF protein expression of CAPD rat PMC, and the increase is followed by increase of glucose concentrations of PDF. Hyper glucose dialysate in CAPD rats may lead to peritoneum ultrafiltration failure by promoting the expression of VEGF,and impair the mesothelial cells.

关 键 词：腹膜透析 血管内皮生长因子 模型/动物 大鼠 

分 类 号：R36-33[医药卫生—病理学]