大黄对大鼠肝再生过程中cyclinD_1影响的实验研究  

Experimental study of the effects of Rhubarb on cyclinD1 during liver regeneration in rats

作　　者：李天兴[1] 朱清静[1] 蔡艳萍[1] 周星[1] 阮连国[1] 李霞[1] 万十千[1] 

机构地区：[1]武汉市医疗救治中心,湖北武汉430032

出　　处：《中国中西医结合消化杂志》2010年第4期227-229,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：湖北省自然科学基金课题资组助(2009CDB129)

摘　　要：[目的]探讨大黄对大鼠肝再生过程中cyclin D1的影响。[方法]将24只Wistar大鼠随机分为4组,分别为对照组、模型组、大黄组和促肝细胞生长素(pHGF)组,每组6只。模型组、大黄组和pHGF组大鼠切除肝脏左叶和中叶,对照组仅切开腹腔翻动肝左叶和中叶。大黄组和pHGF组于实验前3 d至实验结束,分别腹腔注射大黄1 ml/100g和pHGF 1 ml/100 g,每日1次。术后48 h处死大鼠,应用逆转录聚合酶链反应法检测大鼠肝组织cy-clinD1 mRNA的表达。[结果]与对照组比较,模型组、大黄组和pHGF组大鼠肝组织cyclinD1 mRNA表达均显著升高(P<0.01);与模型组比较,大黄组和pHGF组大鼠肝组织cyclinD1 mRNA表达均显著升高(P<0.01),大黄组和pHGF组大鼠肝组织cyclinD1 mRNA表达差异无统计学意义(P>0.05)。[结论]大黄显著促进cyclinD1 mR-NA表达,从而促进肝部分切除术后大鼠的肝再生。[Objective]To investigate the effects of Rhubarb on cyclinD1 during liver regeneration after partial hepatectomy（PH）in rats and study the mechanisms of Rhubarb on liver regeneration after partial hepatectomy in rats.[Methods]Twenty-four Wistar rats were randomly divided into control,model,Rhubarb and pHGF group,six rats in each group.Left and middle lobes of rat livers in model,Rhubarb and pHGF group were resected.Rat bellies in control group were cut open and left and middle liver lobes were stirred.Rats in Rhubarb and pHGF group were intraperitoneally injected with Rhubarb（1ml/100g）and pHGF（1ml/100g）respectively,once per day,from 3 days before the experiment to the end.All rats were sacrificed at the end of 48th hour after the operations.The expression of cyclinD1mRNA in the livers was detected by RT-PCR.[Results]Compared with control group,the expression of liver cyclinD1 mRNA in model,Rhubarb and pHGF group rats was markedly increased（P〈0.01）;compared with model group,the expression of liver cyclinD1 mRNA in Rhubarb and pHGF group rats was markedly increased（P〈0.01）.There was no significant difference in the expression of liver cyclinD1 mRNA between Rhubarb and pHGF group（P〈0.05）.[Conclusion]Rhubarb can significantly promote the expression of cyclinD1 mRNA in the liver of PH rats,and thus enhance liver regeneration in rats after PH.

关 键 词：CYCLIND1 肝再生 大黄 

分 类 号：R575[医药卫生—消化系统] R285.5[医药卫生—内科学]