清胆胶囊对胆固醇结石小鼠肝脏中PPAR-γ、CYP7A1及NF-κB表达的影响  

Effects of Qingdan capsule on PPAR-γ,CYP7A1 and NF-κB of liver in mice with cholesterol gallstone

作　　者：张静喆[1] 梁晓强[2] 顾宏刚[1] 章学林[1] 

机构地区：[1]上海中医药大学附属龙华医院普通外科,上海200032 [2]上海中医药大学附属龙华医院中医外科研究所,上海200032

出　　处：《中国中西医结合消化杂志》2010年第4期254-256,259,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：上海市教委创新团队资助课题;上海市医学领军人才资助课题(No:LJ06048);上海市科委自然科学基金项目(No:09ZR1432300)

摘　　要：[目的]观察清胆胶囊对胆固醇结石小鼠肝脏过氧化物酶体增殖物激活受体(PPAR)-γ、胆固醇7α-羟化酶(CYP7A1)及核因子(NF)-κB表达的影响。[方法]38只C57BL/6雌性小鼠随机分为正常对照组(n=10)、模型组(n=15)和清胆胶囊组(n=13)。除正常对照组外,模型组和清胆胶囊组小鼠采用高脂饮食诱发法建立胆固醇结石模型。造模过程中,清胆胶囊组小鼠予清胆胶囊0.36 g/(kg.d)灌胃治疗。8周后观察各组小鼠的成石率,并用Western Blotting法检测肝脏中PPAR-γ、CYP7A1及NF-κB的表达。[结果]清胆胶囊组成石率显著降低(P<0.01),小鼠肝脏PPARγ及CYP7A1表达增强,NF-κB表达降低。[结论]清胆胶囊可通过增强肝脏PPARγ及CYP7A1的表达,抑制NF-κB核转位来发挥防治胆固醇结石的作用。[Objective]To observe the effects of Qingdan capsule（QC）on liver perosisome proliferator activated receptor gamma（PPAR-γ）,cholesterol 7 alpha-hydroxylase（CYP7A1）and nuclear factor-kappa B（NF-κB）in C57BL/6J mice model with cholesterol gallstone.[Methods]Thirty-eight C57BL/6J mice were randomly divided into normal control group（n=10）,model group（n=15）and QC group（n=13）.Cholesterol gallstone model was induced by feeding high-cholesterol diet.Mice in the QC group were intragastricly administered QC 0.22g/（kg d）.After 8-week treatment,animals were sacrificed and sampled to calculate the incidences of stone formation.The expression of PPAR-γ,CYP7A1 and NF-κB in liver tissues were detected with western blotting.[Results]The incidence rate of stone formation was 73.33% in the model group,0% in normal control group,and 23.08% in the QC group.The incidence rate in the model group was significantly higher than the normal control group and the QC group（P〈0.01）.The expressions of PPAR-γ and CYP7A1 were up-regulated while the expression of NF-κB was down-regulated after the treatment of QC.[Conclusion]QC can decrease the incidence rate of stone formation,induce PPAR-γ and CYP7A1,and decrease NF-κB,which may be its important mechanisms in the treatment and prevention of cholesterol gallstone disease.

关 键 词：清胆胶囊 胆固醇结石 小鼠 过氧化物酸体增殖物激活受体γ 胆固醇7Α-羟化酶 核因子-κB 

分 类 号：R575.62[医药卫生—消化系统] R285.5[医药卫生—内科学]