植物内生真菌SIPI3.0550的分类鉴定及代谢产物研究  

Identification of endophytic fungi SIPI3.0550 and its metabolites

作　　者：张志杰[1,2] 屈凌波[1] 周雨朦[2] 李继安[2] 邵雷[2] 陈代杰[2] 

机构地区：[1]河南工业大学生物工程学院,郑州450001 [2]上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国新药杂志》2010年第16期1402-1406,共5页Chinese Journal of New Drugs

基　　金：重大新药创制科技重大专项(2009ZX09301-007)

摘　　要：目的:对植物内生真菌SIPI3.0550进行分类鉴定,并研究其代谢产物。方法:采用固体发酵方法进行培养;检测发酵产物的抗革兰阳性菌(Gram-positive bacteria,G+)、革兰阴性菌(Gram-negative bacteri-a,G-)和真菌活性;使用溶剂萃取、大孔吸附树脂柱层析和结晶等方法分离其中的脂溶性成分;并通过理化性质及光谱分析鉴定其化学结构。结果:从其代谢产物中分离得到两个化合物monomethylsulochrin(1)和trypacidin(2);活性检测结果显示化合物2有抗G+菌和甲氧西林耐药金黄色葡萄球菌(Methicillin-resistant Staphylococcus aureus,MRSA)的活性。结论:从植物内生真菌SIPI3.0550中分离得到抗菌活性成分,并发现其有较高的抗MRSA活性。Objective： To identify the endophytic fungi SIPI3.0550 and to study its metabolites.Methods： Solid-state fermentation was used to culture the endophytic fungi.The activity of the fermentation extracts against Gram-positive bacteria（G＋）,Gram-negative bacteria（G-） and fungi was determined.The methods of solvent extraction,macroporous resin column chromatography and crystallization were used to separate and purify the liposoluble components of SIPI3.0550.Structures of the purified compounds were elucidated by physico-chemical properties and spectral analysis.Results： Two compounds were separated and identified,i.e.,monomethylsulochrin（1） and trypacidin（2）.The compound 2 showed the activity against G＋ and Methicillin-resistant Staphylococcus aureus（MRSA）.Conclusion： Bioactive metabolites with a higher anti-MRSA activity are obtained from endophytic fungi SIPI3.0550.

关 键 词：植物内生真菌 固体发酵 monomethylsulochrin trypacidin 抗菌活性 

分 类 号：R962[医药卫生—药理学] R978.5[医药卫生—药学]