^(125)Ⅰ标记抗C-erbB-2单抗对荷人乳腺癌裸鼠的定位诊断及生物学分布  

Radioimmunolocalization and biodistribution of ^(125)Ⅰ labeled Anti-C-erbB-2 monoclonal antibody ICR12 in nude mice bearing human breast cancer xenografts

作　　者：杨金巧[1] 王琳[1] 龙启明[1] 胡锐 周蕾蕾[1] 赵俊玲[1] 范伟 

机构地区：[1]华西医科大学附属第一医院普外科,成都610041

出　　处：《中华实验外科杂志》1999年第3期246-247,共2页Chinese Journal of Experimental Surgery

摘　　要：目的研究125I标记抗C-erbB-2单克隆抗体ICR12（简称125I-ICR12）及正常小鼠IgG（简称125I-mIgG）在荷入乳腺癌探鼠体内的分布，为放射免疫显像（RII）及临床应用提供依据。方法每鼠尾静脉注射抗体92．5KBq／0．1ml，于注射后24、48、96、120／小时分批处死，测定肿瘤和血液、肝、肺等重要脏器的单位重量放射性比值（T／NT）、各组织摄取百分比（％ID／g）及定位指数（LI）。结果125I-ICR12注射后24～120／小时内，肿瘤部位出现选择性放射性浓聚；T／NT在120／小时最高，为19．374。125I-mlgG未出现放射性浓聚，呈全身分布。结论ICR12在荷人乳腺癌裸鼠体内，对过度表达C-erbB-2的乳腺癌具有亲和力，可望用作乳腺癌诊断、预后判断的导向载体。Objective To study the distribution of12s I labeled anti-C-erbB-2 monoclonal antibodyICR12 (125I-ICR12) and normal mice IgG in nude rnice bearing human breast cancer and to provide thebasis for radioimmunoimaging (RII) and clinical use. Methods. 92. 5 KBq/0. 1 ml labeled antibodywas injected into the tail vein in each mouse respectively. Radioactivity ratio of tumor and non-tumor(T/NT), percent injected dosage of every gram tissue (%ID/g), and localization index (LI) were measured after 24, 48, 98, 120 h interval of injection. Results Radioactivity concentration in tumor wasfound during the 24 to 120 h after the 125I-ICR12 injection. The peak value of T/NT was 19. 374 at 120h, but non-concentration of 125I-mIgG was found, which was distributed evenly in body. ConclusionsICR12 had affinity to breast cancer which overexpressed C-erbB-2 in nude mice bearing human breastcancer xenografts. ICR12 is prospective to be used as the direct carrier of breast cancer diagnosis.[

关 键 词：乳腺癌 C-ERBB-2 单克隆抗体 碘125 

分 类 号：R737.9[医药卫生—肿瘤]