外源性神经节苷脂对小鼠巨噬细胞抗肿瘤相关细胞因子表达的影响  

EFFECTS ON THE CYTOKINE EXPRESSION OF MOUSE MACROPHAGES BY EXOGENOUS GANGLIOSIDES

作　　者：吴欣[1] 刘银坤[1] 张夏英[1] 

机构地区：[1]上海医科大学肝癌研究所

出　　处：《中国癌症杂志》1999年第2期138-140,共3页China Oncology

基　　金：国家自然科学基金

摘　　要：目的在转录水平上研究外源性神经节苷脂（ＧＭ３）、（ＧＤ３）对小鼠巨噬细胞抗肿瘤相关因子白细胞介素１β（ＩＬ１β）、肿瘤坏死因子α（ＴＮＦα）、白细胞介素６（ＩＬ６）表达的影响。方法用反转录聚合酶链反应（ＲＴＰＣＲ）法和密度扫描半定量ＰＣＲ产物，比较测定细胞因子ＩＬ１β、ＴＮＦα、ＩＬ６ｍＲＮＡ（信使核糖核酸）表达量。结果单唾液酸神经节苷脂ＧＭ３、双唾液酸神经节苷脂ＧＤ３对巨噬细胞作用２４ｈ后，与未处理组比较，ＧＭ３可极大地增加ＩＬ１β、ＩＬ６、ＴＮＦαｍＲＮＡ的表达，分别在６ｍｇ／Ｌ、４ｍｇ／Ｌ和４ｍｇ／Ｌ时使３种细胞因子增加１３６、８３和４６４倍。ＧＤ３对ｍＲＮＡ的表达仅见微弱调节作用。结论ＧＭ３可在转录水平上对上述３种细胞因子进行正调控，且与剂量呈相关性，而ＧＤ３则基本上无作用。一定程度上反映了ＧＭ３、ＧＤ３对巨噬细胞免疫功能的影响。PURPOSE To study the effects of certain ganaliosides on the modulation of the expression of IL 1β、TNFα and IL 6 of mouse macrophage in transcripal level in vitro. METHODS The mRNA transcripts of IL 1、IL 6 and TNF of macrophages stimulated by GM3 and GD3 were detected by semiquantitative reverse transcription polymerase chain reaction(RT PCR). RESULTS Comparing with the control,GM3 could increase the expression of IL 1β、IL 6 and TNFα greatly.The most effective concentrations were 6 mg/L,4 mg/L and 4 mg/L respectively,the increasing folds for these three cytokines were up to 13.6,8.3 and 46.4 However,GD3 either had no effect or only slightly up regulated the expression of these three cytokines. CONCLUSIONS The results metioned above indicate that GM3 can up regulate these three cytokines in transcription level in a dose dependent manner.In contrast,GD3 have no obvious effects on cytokin expression.The alternations of these three cytokines may suggest the influence of the imunological function of mouse marcrophages by GM3 or GD3.

关 键 词：神经节苷脂 巨噬细胞 细胞因子 RT-PCR 抗肿瘤 

分 类 号：R730.3[医药卫生—肿瘤] R392.11[医药卫生—临床医学]