预防仔猪腹泻无抗性基因工程菌株的构建  

CONSTRUCTION OF A NONE-ANTIBIOTIC RESISTANT ETEC VACCINE CANDIDATE STRAIN EXPRESSING K88ac AND LT-B ANTIGENS

作　　者：黄培堂[1] 程军 李丰生[1] 徐香[1] 张群伟 陈添弥[1] 黄翠芬[1] 

机构地区：[1]军事医学科学院生钧工程研究所

出　　处：《生物工程学报》1990年第1期11-17,共7页Chinese Journal of Biotechnology

摘　　要：以原来含热不稳定肠毒素B亚单位基因质粒DNA为载体,插入lacZ基因,构建了一个既能表达LT-B亚单位又能表达β-半乳糖苷酶的含四环素抗性基因的重组体。在该重组DNA的四环素抗性基因内再插入K88粘附因子抗原基因,从而灭活了四环素抗性基因。经测定该重组体能表达LT-B和K88两种抗原;lacZ基因取代四环素抗性基因,成为良好筛选标记。所构建的重组质粒能稳定存在。By inserting the lacZ gene into a plasmid pPMC21 containing ETEC LT-B gene a recombinant plasmid pMM101 was obtained, which could express β-galactosidase and LT-B antigen. But pMM101 has still Tc resistant gene. K88ac gene was then inserted into BamH Ⅰ site of Te resistant gene region in pMM101. The final recombinant plasmid, pMM103, has not carried any antibiotic resistance gene but can stably express both LT-B and K88ac antigens, and also the β-galactosidase as attacking marker. The strain carring plasmid pMM103 (MM6) was used to immune the animal. The results have indicated that MM-6 can effectively protect the animal from the attack of the ETEC bacteria, and can be used as candidate of live vaccine strain.

关 键 词：仔猪腹泻 抗性基因 基因表达 

分 类 号：S188[农业科学—农业基础科学]