液相色谱串联质谱法用于肌酸的长期毒性研究  

Investigation of Potential Toxicity of Chronic Creatine Ingestion by HPLC-ESI-MS

作　　者：王琳[1] 张永谦[1] 车宝泉[1,2] 肖盛元[1] 邓玉林[1] 

机构地区：[1]北京理工大学生命学院,北京100081 [2]北京市药品检验所,北京100035

出　　处：《化学通报》2010年第9期846-849,共4页Chemistry

基　　金：十一五科技支撑项目(2006BAK03A07)资助

摘　　要：肌酸是一种普遍流行的能量增强剂,在生物体内代谢为甲胺,甲胺在对氨基脲敏感的胺氧化酶(semicarbazied-sensitive amine oxidases,SSAO)的催化下进一步生成甲醛、过氧化氢和氨。本文将肌酸的毒性与SSAO活性的升高和产物甲醛的积累联系起来,提出肌酸代谢的毒性假说,建立液相色谱串联离子阱质谱检测SSAO活性的新方法。结果表明,服用肌酸2个月后小鼠尿液中甲胺、甲醛含量升高,血清中SSAO活性升高,对肌酸的长期毒性给于了肯定的评价。Creatine is alleged to be a popular nutrition to enhance sports performance.It can be metabolized to methylamine,which is further converted to formaldehyde,hydrogen peroxide and ammonium by semicarbazied-sensitive amine oxidases（SSAO）.Potential toxicity of chronic creatine administration was discussed by the increase of SSAO activity and toxic products.A novel HPLC-ESI-MS method for the determination of SSAO activity was developed.In this study,we demonstrated that after chronic administration of creatine to mice,the level of methylamine and formaldehyde in urine were increased,SSAO activity of serum was increased simultaneously.Although creatine is a major endogemous constituent,chronic creatine ingestion in a large dose as a nutrition supplement would be potentially toxicity.

关 键 词：肌酸 SSAO 毒性 HPLC-ESI-MS 

分 类 号：R114[医药卫生—卫生毒理学]