鳖甲煎丸含药大鼠血清对人肝癌HEPG2细胞Mg^(2+)-ATP酶、G-6-P酶活性影响的实验研究  

The Rat's Blood Serum of Biejia Decoction for Activity of Human'S Liver HEPG2 Cell Mg^(2+)- ATP Enzyme and G - 6 - P Enzyme to Study

作　　者：王丹[1] 宋昊[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032

出　　处：《实用中医内科杂志》2010年第7期20-21,共2页Journal of Practical Traditional Chinese Internal Medicine

摘　　要：[目的]研究鳖甲煎丸含药大鼠血清对人肝癌HEPG2细胞Mg^(2+)-ATP酶、G-6-P酶活性的影响。[方法]将实验大鼠随机分为4组:空白对照组、鳖甲煎丸煎剂组、顺铂组、鳖甲煎丸联合顺铂用药组。待人肝癌HEPG2细胞贴壁生长状态良好,加入含药大鼠血清及对照组大鼠血清,培养后取材制作标本。利用Mg^(2+)-ATP酶、G-6-P酶细胞化学染色方法对标本进行染色。运用光镜观察酶反应。[结果]光镜下,鳖甲煎丸煎剂组、顺铂组、鳖甲煎丸联合顺铂用药组Mg^(2+)-ATP酶、G-6-P酶活性减弱,与空白对照组相比较有明显差异,差别有统计学意义(P<0.05)。[结论]鳖甲煎丸含药大鼠血清对人肝癌HEPG2细胞Mg^(2+)-ATP酶、G-6-P酶活性的影响有减弱作用。[ Objective] Discuss the effect of the rat＇ s blood serum of Biejia decoction for activity of human＇ s liver HIPG2 cell Mg2＋-ATP enzyme and G -6 -P enzyme. [ Methods ] The rats for experiment were separated as physiological saline group, Biejia decoction group, DDP group, Biejia decoction and DDP group. When human＇ s liver HEPG2 cells were in good condition, including the serum of the rat each group. After cultu- ring, make the specimen. To observe the rats＇ serum with the micro - scope is after making the specimen chemical coloring process. [ Results ] Under the micro -scope, the Mg2＋ -ATP enzyme and G -6 -P enzyme activity are all decreased of Biejia decoction group, DDP group, Biejia decoction and DDP. Have the marked difference with the physiological saline group （ P 〈 0.05 ）. [ Conclusion ] The activity of human＇ s liver HIPG2 cell Mg2＋ -ATP enzyme and G-6 -P enzyme are decreased with Biejia decoction.

关 键 词：人肝癌HEPG2细胞 Mg2^+-ATP酶 C-6-P酶 鳖甲煎丸 

分 类 号：R-33[医药卫生]